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Contributo in rivista
Tipo: Articolo in rivista
Titolo: Expression profile of genes coding for DNA repair in human oocytes using pangenomic microarrays, with a special focus on ROS linked decays.
Anno di pubblicazione: 2007
Formato: Elettronico Cartaceo
Autori: Menezo Y, Russo GL, Tosti E, El-Mouatassim S, Benkhalifa M.
Affiliazioni autori: Istituto di Scienze dell'Alimentazione CNR Avellino Stazione Zoologica "A. Dohrn" Napoli UNILABS, 12 Place Cornavin, Geneva, Switzerland Laboratoire d'Eylau, 75116 Paris, France
Abstract: Purpose To determine the level of expression for mRNAs that regulate DNA repair activity in oocytes at the germinal vesicle (GV) stage. Reactive oxygen species (ROS) have been shown to play a major role in the appearance of deleterious DNA decays, and this study focuses on the repair of damage linked to decay caused by the action of ROS. The oocyte needs a mechanism for repairing DNA decays in the early preimplantation embryo before the onset of genomic activation, since in the absence of repair, residual DNA damage would lead to either apoptosis or tolerance. Tolerance of DNA damage is a source of potential mutations. Method GV oocytes were selected for this study, both for the ethical reason that they are unsuitable for patient treatment, and because no transcription takes place during the period from GV to MII and then prior to genomic activation. The GV oocyte is therefore a good model for looking at DNA during the first cleavages of early preimplantation development. Six cohorts of GV oocytes were pooled for extraction of mRNA; the DNA was analysed using Affimetrix HG-UG133 Plus 2, containing 54,675 probe sets; spike and housekeeping genes were also added as internal controls. Results In GV oocytes, DNA repair pathways for oxidized bases are redundant. One step repair procedure (OSR), BER (base excision repair), MMR (mismatch repair) and NER (Nucleotide excision repair) are present. All the recognition proteins are also present. The chromatin assembly factors necessary for the maintenance of genomic stability are highly expressed. Conclusion Gene expression analysis shows that the oocyte does not allow a high level of tolerance for DNA decays. This regulatory mechanism should avoid transmitting mutations into the next generation.
Lingua abstract: inglese
Pagine da: 513
Pagine a: 520
Journal of assisted reproduction and genetics
Numero volume: 24
Numero fascicolo: 11
Referee: Sė: Internazionale
Indicizzato da: PubMed 
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