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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2008

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Molecular modelling of miraculin: Structural analyses and functional hypotheses.

Anno di pubblicazione: 2008

Autori: Paladino A, Costantini S, Colonna G, Facchiano AM.

Autori CNR:

  • SUSAN COSTANTINI
  • ANGELO FACCHIANO

Abstract: Miraculin is a plant protein that displays the peculiar property of modifying taste by swiching sour into a sweet taste. Its monomer is flavourless at all pH as well as at high concentration; the dimer form elicits its taste-modifying activity at acidic pH; a tetrameric form is also reported as active. Two histidine residues, located in exposed regions, are the main responsible of miraculin activity, as demonstrated by mutagenesis studies. Since structural data of miraculin are not available, we have predicted its three-dimensional structure and simulated both its dimer and tetramer forms by comparative modelling and molecular docking techniques. Finally, molecular dynamics simulations at different pH conditions have indicated that at acidic pH the dimer assumes a widely open conformation, in agreement with the hypotheses coming from other studies.

Pagine da: 26

Pagine a: 32

Rivista:

Biochemical and biophysical research communications Elsevier [etc.]
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0006-291X

Numero volume: 367

Altre informazioni: 0

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