Home |  English version |  Mappa |  Commenti |  Sondaggio |  Staff |  Contattaci Cerca nel sito  
Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2007

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Gastrokine 1 expression in patients with and without Helicobacter pylori infection.

Anno di pubblicazione: 2007

Autori: Nardone G; Rippa E; Martin G; Rocco A; Siciliano RA; Fiengo A; Cacace G; Malorni A; Budillon G; Arcari P.

Affiliazioni autori: Dipartimento di Medicina Clinica e Sperimentale, Unità di Gastroenterologia, Universit`a di Napoli Federico II, Naples, Italy Dipartimento di Biochimica e Biotecnologie Mediche, Università di Napoli Federico II, Naples, Italy Centro di Spettrometria di Massa Proteomica e Biomolecolare, Istituto di Scienze dell’Alimentazione—CNR, Avellino, Italy CEINGE Biotecnologie Avanzate Scarl, Naples, Italy

Autori CNR:

  • GIUSEPPINA CACACE
  • ANTONIO MALORNI
  • ROSA ANNA SICILIANO

Lingua: inglese

Abstract: BACKGROUND: To understand the molecular changes underlying Helicobacter pylori-related gastric diseases is mandatory to prevent gastric cancer. Proteomic technology is providing a rapid expansion of the basic knowledge, particularly in the discovery of new biomarkers involved in the tumourigenesis. AIM: To characterise changes in protein expression level of the gastric mucosa in H. pylori-infected patients. METHODS: The population enrolled comprised 41 dyspeptic patients. Proteins extracted from gastric mucosal specimens were analysed by 2-dimensional electrophoresis, sequenced by MALDI-TOF and identified by Edman's degradation. RESULTS: Twenty-one out of 41 patients had H. pylori infection of whom 17 had anti-CagA IgG antibodies. Several proteins were identified, of which Rho guanosine diphosphatase dissociation inhibitor alpha and heat shock protein 27 increased and glutathione transferase and antrum mucosa protein-18 decreased in H. pylori-positive in respect to H. pylori-negative patients. Interestingly, antrum mucosa protein-18, currently referred as gastrokine-1, showed two isoforms differing in the first N-terminal amino acid residue. Both gastrokine-1 isoforms were observed in the H. pylori-negative group whereas a lower expression or even absence of the gastrokine-1 basic isoform was found in a subgroup (7/21) of H. pylori-positive patients with moderate-severe gastritis. CONCLUSION: Our study demonstrated the presence of gastrokine-1 isoforms of which the basic isoform was reduced in a subset of patients with H. pylori infection.

Lingua abstract: inglese

Pagine da: 122

Pagine a: 129

Pagine totali: 8

Rivista:

Digestive and Liver Disease Editrice Gastroenterologica Italiana.
Paese di pubblicazione: Italia
Lingua: inglese
ISSN: 1590-8658

Numero volume: 39

DOI: 10.1016/j.dld.2006.09.017

Referee: Sì: Internazionale

Indicizzato da:

  • ISI Web of Science (WOS) [000244930000003]
  • PubMed [17092786]

Parole chiave:

  • AMP-18
  • Chronic gastritis
  • Gastrokine-1
  • H. pylori
  • Post-translational modification

Strutture CNR:

Moduli:

 
Torna indietro Richiedi modifiche Invia per email Stampa
Home Il CNR  |  I servizi News |   Eventi | Istituti |  Focus