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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2007

Contributo in rivista

Tipo: Articolo in rivista

Titolo: APEX/Ref-1 (Apurinic/Apyrimidic Endonuclease DNA-Repair Gene) Expression in Human and Ascidian (Ciona intestinalis) Gametes and Embryos.

Anno di pubblicazione: 2007

Formato: Elettronico Cartaceo

Autori: El-Mouatassimi S, Bilotto S, Russo GL, Tosti E, Menezo Y.

Affiliazioni autori: Laboratoire Marcel Me´rieux, Avenue Tony Garnier, Lyon, France Stazione Zoologica 'Anton Dohrn', Napoli, Italy; National Research Council, Avellino, Italy; Laboratoire d'Eylau/Unilabs, 55 Rue Saint Didier, 75116, Paris

Autori CNR:

  • GIAN LUIGI RUSSO

Lingua: inglese

Abstract: In recent years, the impact of sperm DNA damage on fertility has become an important issue. The different technologies developed to check sperm DNA fragmentation lead to the same conclusion: DNA damage negatively impacts upon reproductive processes. Oocyte DNA repair capacity is one of the cues to understanding embryo developmental arrest. APEX/Ref-1 (apurinic/apyrimidic endonuclease) is an enzyme involved in the DNA base excision repair pathway removing the abasic sites, the most common DNA decays. In humans, APEX has a multifunctional role, including the control of the redox status of transcription factors. RT-PCR allowed us to detect human APEX transcripts in oocytes, spermatozoa and preimplantation blocked embryos. In parallel, a comparative study on sea squirt Ciona intestinalis (ascidian) indicated that APEX transcripts are clearly detectable in oocytes and embryos until the larva stage, but not in spermatozoa, suggesting the appearance of the paternal contribution to DNA repair during development having arisen only late in Vertebrate evolution. Of additional phylogenetic significance is the observation that sea squirt APEX appears to lack redox transcriptional activity.

Lingua abstract: inglese

Pagine da: 549

Pagine a: 556

Rivista:

Molecular human reproduction Oxford University Press,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1360-9947

Numero volume: 13

Numero fascicolo: 8

DOI: 10.1093/molehr/gam038

Referee: Sì: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000248848100015]

Altre informazioni: Free article at: http://molehr.oxfordjournals.org/content/13/8/549.full.pdf+html

Strutture CNR:

Moduli:

Allegati: Mol. Hum. Reprod.-2007-El-Mouatassim-549-56 (application/pdf)

 
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