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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2007

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Identification of gadoid species (Pisces, Gadidae) by sequencing and PCR-RFLP analysis of mitochondrial 12S and 16S rRNA gene fragments.

Anno di pubblicazione: 2007

Formato: Elettronico Cartaceo

Autori: Di Finizio A, Guerriero G, Russo GL, Ciarcia G.

Affiliazioni autori: Section of Zoology, Department of Biological Sciences, University of Naples "Federico II", Naples Institute of Food Sciences, National Research Council, Avellino

Autori CNR:


Lingua: inglese

Abstract: We applied PCR-RFLP (PolymeraseChainReaction - Restriction Fragment Length Polymorphism) analysis to identify seven gadoid species of different biogeographical origin and commercial relevance, namely Gadus morhua (Atlantic ocean); Trisopterus minutus capelanus, Trisopterus minutus minutus, Molva elongata, Phycis blennoides, Micromesistius poutassou (Atlantic ocean and Mediterranean sea); Theragra chalcogramma (Pacific ocean). Two DNA fragments belonging to mitochondrial 12S and 16S rRNA genes of about 430 and 630 bp, respectively, were isolated by PCR amplification. Their direct sequencing showed a significant genotypic diversity among gadoid species, useful for species identification. Digestion of 16S rRNA gene PCR fragment with MvaI or Bsh1285I restriction enzymes, followed by agarose gel electrophoresis of the cleaved products, yielded specific restriction profiles that enabled direct, visual identification of the species analyzed. This PCR- RFLP method allowed a clear and rapid discrimination of the gadoid species studied.

Pagine da: 337

Pagine a: 344


European food research & technology Springer
Paese di pubblicazione: Germania
Lingua: inglese
ISSN: 1438-2377

Numero volume: 225

DOI: 10.1007/s00217-006-0420-z

Referee: Sė: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000247502000005]

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