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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2007

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Regulatory roles of nitric oxide during larval development and metamorphosis in Ciona intestinalis.

Anno di pubblicazione: 2007

Formato: Elettronico Cartaceo

Autori: Comes S, Locascio A, Silvestre F, d'Ischia M, Russo GL, Tosti E, Branno M, Palumbo A.

Affiliazioni autori: Stazione Zoologica "Anton Dohrn", Naples, Italy Department of Organic Chemistry and Biochemistry, University of Naples "Federico II", Naples, Italy Institute of Food Sciences, National Research Council, Avellino

Autori CNR:

  • GIAN LUIGI RUSSO

Lingua: inglese

Abstract: Metamorphosis in the ascidian Ciona intestinalis is a very complex process which converts a swimming tadpole to an adult. The process involves reorganisation of the body plan and a remarkable regression of the tail, which is controlled by caspase-dependent apoptosis. However, the endogenous signals triggering apoptosis and metamorphosis are little explored. Herein, we report evidence that nitric oxide (NO) regulates tail regression in a dose-dependent manner, acting on caspase-dependent apoptosis. An increase or decrease of NO levels resulted in a delay or acceleration of tail resorption, without affecting subsequent juvenile development. A similar hastening effect was induced by suppression of cGMP-dependent NO signalling. Inhibition of NO production resulted in an increase in caspase-3-like activity with respect to untreated larvae. Detection of endogenously activated caspase-3 and NO revealed the existence of a spatial correlation between the diminution of the NO signal and caspase-3 activation during the last phases of tail regression. Real-time PCR during development, from early larva to early juveniles, showed that during all stages examined, NO synthase (NOS) is always more expressed than arginase and it reaches the maximum value at late larva, the stage immediately preceding tail resorption. The spatial expression pattern of NOS is very dynamic, moving rapidly along the body in very few hours, from the anterior part of the trunk to central nervous system (CNS), tail and new forming juvenile digestive organs. NO detection revealed free diffusion from the production sites to other cellular districts. Overall, the results of this study provide a new important link between NO signalling and apoptosis during metamorphosis in C. intestinalis and hint at novel roles for the NO signalling system in other developmental and metamorphosis-related events preceding and following tail resorption.

Pagine da: 772

Pagine a: 784

Rivista:

Developmental biology Academic Press : San Diego [etc.]
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0012-1606

Numero volume: 306

Referee: Sė: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000247459900027]

Altre informazioni: Accesso libero al sito: http://www.sciencedirect.com/science/article/pii/S0012160607007907

Strutture CNR:

Moduli:

Allegati: Developmental Biology 306 (2007) 772-784 (application/pdf)

 
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