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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2010

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Quercetin induced apoptosis in association with death receptors and fludarabine in cells isolated from chronic lymphocytic leukaemia patients

Anno di pubblicazione: 2010

Formato: Elettronico Cartaceo

Autori: Russo, M., Spagnuolo, C. Volpe, S. Mupo, A. Tedesco, I. Russo, G. L.

Affiliazioni autori: Istituto Scienze dell'Alimentazione Ospedale Moscati Avellino

Autori CNR:


Abstract: BACKGROUND: Quercetin is a flavonoid naturally present in food and beverages belonging to the large class of phytochemicals with potential anti-cancer properties. Here, we investigated the ability of quercetin to sensitise primary cells from chronic lymphocytic leukaemia (CLL) to death receptor (DR) agonists, recombinant TNF-related-apoptosis-inducing ligand (rTRAIL) and anti-CD95, and to fludarabine, a widely used chemotherapeutic drug against CLL. METHODS: Peripheral white blood cells were isolated from patients and incubated with medium containing 50 ng ml anti-CD95 agonist antibody; 10 ng ml recombinant TRAIL; 10-25 microM quercetin and 3.5-14 microM fludarabine. Neutral Red assay was used to measure cell viability, where as apoptosis was assessed by determining caspase-3 activity, exposure to Annexin V and PARP fragmentation. RESULTS: Quercetin significantly enhanced anti-CD95- and rTRAIL-induced cell death as shown by decreased cell viability, increased caspase-3 and -9 activities, and positivity to Annexin V. In addition, association of quercetin with fludarabine increases the apoptotic response in CLL cells of about two-fold compared with quercetin monotreatment. CONCLUSION: This work shows that resistance to DR- and fludarabine-induced cell death in leukaemic cells isolated from CLL patients can be ameliorated or bypassed by the combined treatment with quercetin. Considering the low toxicity of the molecule, our study results are in favour of a potential use of quercetin in adjuvant chemotherapy in combination with other drugs.

Lingua abstract: inglese

Pagine da: 642

Pagine a: 648


British Journal of Cancer H K Lewis and Co, Ltd.
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0007-0920

Numero volume: 103

Referee: Sė: Internazionale

Indicizzato da:

  • PubMed [20648016]
  • ISI Web of Science (WOS) [000281287700007]

Parole chiave:

  • chronic lymphocytic leukaemia
  • quercetin
  • CD95
  • fludarabine

Altre informazioni: Press release: http://www.stampa.cnr.it/documenti/comunicati/italiano/2010/luglio/55_lug_2010.htm

Strutture CNR:


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