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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2006

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Assessment of the conformational features of vasoactive intestinal peptide in solution by limited proteolysis experiments.

Anno di pubblicazione: 2006

Autori: Stiuso P., Marabotti A., Facchiano A., Lepretti M., Dicitore A., Ferranti P., Carteni M.

Affiliazioni autori: Seconda UniversitÓ di Napoli Istituto di Scienza dell'Alimentazione, CNR, Avellino

Autori CNR:

  • ANGELO FACCHIANO
  • ANNA MARABOTTI
  • ANNA MARABOTTI

Abstract: The structural features of vasoactive intestinal peptide (VIP) and of its Gln16-diaminopropane derivative (VIP-DAP) in solution were investigated by limited proteolysis experiments with trypsin and thermolysin. The proteolysis of the native peptide by both proteinases takes place near the residues in positions 12 and 21/22, suggesting that these amino acids are embedded in segments more flexible than the rest of the molecule. VIP-DAP appears to be more resistant to the proteolytic attack of trypsin, indicating that the derivatization in position 16 is able to stabilize the structure of the peptide. Moreover, the analysis of the mass spectra of the proteolytic mixtures supports the evidence that the derivatization is also able to protect Met17 against oxidation. From these data it can be concluded that VIP in solution under physiological conditions is characterized by the presence of segments with secondary structure, linked together by "hinge" regions that confer flexibility to the peptide, whereas VIP-DAP is embedded in a more rigid conformation, more suitable to receptor interaction.

Pagine da: 110

Pagine a: 119

Rivista:

Biopolymers John Wiley & Sons, etc.]
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0006-3525

Numero volume: 81

DOI: 10.1002/bip.20385

Indicizzato da: PubMed [16224772]

URL: http://onlinelibrary.wiley.com/doi/10.1002/bip.20385/abstract;jsessionid=766CC91195A2AE2A8590304A3CDB418D.d01t02

Altre informazioni: 4

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