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Contributo in rivista
Tipo: Articolo in rivista
Titolo: MALDI-TOF mass spectrometry for the discrimination of foodborne microorganisms
Anno di pubblicazione: 2006
Formato: Elettronico Cartaceo
Autori: Mazzeo MF; Sorrentino A; Gaita M; Cacace G; Di Stasio M; Facchiano A; Comi G; Malorni A and Siciliano RA.
Affiliazioni autori: Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Science and Technology, CNR, Avellino, Italy, and Department of Food Science, University of Udine, Udine, Italy
Abstract: A methodology based on matrix-assisted laser desorption ionization-time of flight mass spectrometry of intact bacterial cells was used for rapid discrimination of 24 bacterial species, and detailed analyses to identify Escherichia coli O157:H7 were carried out. Highly specific mass spectrometric profiles of pathogenic and nonpathogenic bacteria that are well-known major food contaminants were obtained, uploaded in a specific database, and made available on the Web. In order to standardize the analytical protocol, several experimental, sample preparation, and mass spectrometry parameters that can affect the reproducibility and accuracy of data were evaluated. Our results confirm the conclusion that this strategy is a powerful tool for rapid and accurate identification of bacterial species and that mass spectrometric methodologies could play an essential role in polyphasic approaches to the identification of pathogenic bacteria.
Lingua abstract: inglese
Pagine da: 1631
Pagine a: 1638
Applied and environmental microbiology
American Society for Microbiology.
Numero volume: 72
Referee: Sė: Internazionale
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