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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2006

Contributo in rivista

Tipo: Articolo in rivista

Titolo: MALDI-TOF mass spectrometry for the discrimination of foodborne microorganisms

Anno di pubblicazione: 2006

Formato: Elettronico Cartaceo

Autori: Mazzeo MF; Sorrentino A; Gaita M; Cacace G; Di Stasio M; Facchiano A; Comi G; Malorni A and Siciliano RA.

Affiliazioni autori: Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Science and Technology, CNR, Avellino, Italy, and Department of Food Science, University of Udine, Udine, Italy

Autori CNR:

  • GIUSEPPINA CACACE
  • MICHELE DI STASIO
  • ANGELO FACCHIANO
  • MARCELLO GAITA
  • ANTONIO MALORNI
  • MARIA FIORELLA MAZZEO
  • ROSA ANNA SICILIANO
  • ALIDA SORRENTINO

Lingua: inglese

Abstract: A methodology based on matrix-assisted laser desorption ionization-time of flight mass spectrometry of intact bacterial cells was used for rapid discrimination of 24 bacterial species, and detailed analyses to identify Escherichia coli O157:H7 were carried out. Highly specific mass spectrometric profiles of pathogenic and nonpathogenic bacteria that are well-known major food contaminants were obtained, uploaded in a specific database, and made available on the Web. In order to standardize the analytical protocol, several experimental, sample preparation, and mass spectrometry parameters that can affect the reproducibility and accuracy of data were evaluated. Our results confirm the conclusion that this strategy is a powerful tool for rapid and accurate identification of bacterial species and that mass spectrometric methodologies could play an essential role in polyphasic approaches to the identification of pathogenic bacteria.

Lingua abstract: inglese

Pagine da: 1631

Pagine a: 1638

Rivista:

Applied and environmental microbiology American Society for Microbiology.
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0099-2240

Numero volume: 72

DOI: 10.1128/AEM.72.2.1180-1189.2006

Referee: Sė: Internazionale

Indicizzato da:

  • ISI Web of Science (WOS) [000235353100028]
  • PubMed [16461665]

Strutture CNR:

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