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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2004

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Primary structure of water buffalo alpha-lactalbumin variants A and B

Anno di pubblicazione: 2004

Autori: Chianese L., Caira S., Lilla S., Pizzolongo F., Ferranti P., Pugliano G., Addeo F.

Affiliazioni autori: Univ Naples Federico II, Dipartimento Sci Alimenti, Portici, Italy

Autori CNR:

  • SIMONETTA CAIRA

Abstract: A novel electrophoretic alpha-lactalbumin (alpha-la) variant was detected in the Italian water buffalo breed. The isoelectric point of the variant, labelled A, was lower than the most frequent variant B. It presented an allelic frequency of 0(.)5% compared with the 97(.)1% of the BB allele. From Liquid Chromatography-Electrospray Ionization/Mass spectrometry, the molecular mass of the two alpha-la A and B variants were measured as 14235(.)1 +/- 0(.)8 and 14236(.)1 +/- 0(.)9 Da, respectively. The two proteins were sequenced and differentiated from one another by a single amino acid substitution, Asn(45)(B)-->Asp(45)(A). As this amino acid substitution altered the N-glycosylation sequence consensus Asn45-X-Ser(46) it may be deduced that the protein glycosylation level of the alpha-la A would decrease.

Pagine da: 14

Pagine a: 19

Rivista:

Journal of dairy research Empire Marketing Board, Dairy Research Committee,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0022-0299

Numero volume: 71

Parole chiave:

  • aminoacid sequence
  • heterogeneity
  • lysozime
  • protein
  • casein

Altre informazioni: 3

Strutture CNR:

 
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