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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2006

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Gliadin-specific, type 1 regulatory T cell (Tr1) from the intestinal mucosa of treated coeliac disease patients inhibit pathogenic T cell.

Anno di pubblicazione: 2006

Formato: Elettronico

Autori: Gianfrani C, Levings MK, Sartirana C, Mazzarella G, Barba G, Zanzi D, Camarca A, Iaquinto G, Giardullo N, Auricchio S, Troncone R, Roncarolo MG.

Affiliazioni autori: Levings Megan K, San Raffaele Telethon Institute for Gene Therapy (Hospital San Raffaele-Telethon Institute for Gene Therapy). Milan, Italy; & Department of Pediatrics and European Laboratory for the Investigation of Food-Induced Diseases, University Federico II, Naples, Italy; Sartirana Claudia, San Raffaele Telethon Institute for Gene Therapy (Hospital San Raffaele-Telethon Institute for Gene Therapy). Milan, Italy; Zanzi Delia, Department of Pediatrics and European Laboratory for the Investigation of Food-Induced Diseases, University Federico II, Naples, Italy; Camarca Alessandra, istituto di Scienza dell'Alimentazione, CNR Iaquinto Gaetano, Gastroenterology Unit, Moscati Hospital, Avellino, Italy Giardullo Nicola, Gastroenterology Unit, Moscati Hospital, Avellino, Italy Auricchio Salvatore, Department of Pediatrics and European Laboratory for the Investigation of Food-Induced Diseases, University Federico II, Naples, Italy; Troncone Riccardo, Department of Pediatrics and European Laboratory for the Investigation of Food-Induced Diseases, University Federico II, Naples, Italy; Roncarolo Maria Grazia, San Raffaele Telethon Institute for Gene Therapy (Hospital San Raffaele-Telethon Institute for Gene Therapy). Milan, Italy; & Vita-Salute San Raffaele University, Milan, Italy

Autori CNR:

  • GIANVINCENZO BARBA
  • CARMELA GIANFRANI
  • GIUSEPPE MAZZARELLA

Lingua: inglese

Abstract: Celiac disease (CD) results from a permanent intolerance to dietary gluten and is due to a massive T cell-mediated immune response to gliadin, the main component of gluten. In this disease, the regulation of immune responses to dietary gliadin is altered. Herein, we investigated whether IL-10 could modulate anti-gliadin immune responses and whether gliadin-specific type 1 regulatory T (Tr1) cells could be isolated from the intestinal mucosa of CD patients in remission. Short-term T cell lines were generated from jejunal biopsies, either freshly processed or cultured ex vivo with gliadin in the presence or absence of IL-10. Ex vivo stimulation of CD biopsies with gliadin in the presence of IL-10 resulted in suppression of Ag-specific proliferation and cytokine production, indicating that pathogenic T cells are susceptible to IL-10-mediated immune regulation. T cell clones generated from intestinal T cell lines were tested for gliadin specificity by cytokine production and proliferative responses. The majority of gliadin-specific T cell clones had a Th0 cytokine production profile with secretion of IL-2, IL-4, IFN-gamma, and IL-10 and proliferated in response to gliadin. Tr1 cell clones were also isolated. These Tr1 cells were anergic, restricted by DQ2 (a CD-associated HLA), and produced IL-10 and IFN-gamma, but little or no IL-2 or IL-4 upon activation with gliadin or polyclonal stimuli. Importantly, gliadin-specific Tr1 cell clones suppressed proliferation of pathogenic Th0 cells. In conclusion, dietary Ag-specific Tr1 cells are present in the human intestinal mucosa, and strategies to boost their numbers and/or function may offer new therapeutic opportunities to restore gut homeostasis.

Lingua abstract: inglese

Pagine da: 4178

Pagine a: 4186

Pagine totali: 9

Rivista:

The journal of immunology American Association of Immunologists
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 1550-6606

Numero volume: 177

Referee: Sė: Internazionale

Indicizzato da: PubMed [PMID: 16951383]

URL: http://www.jimmunol.org/content/177/6/4178.short

Altre informazioni: 10

Strutture CNR:

Moduli:

Allegati: Gliadin-Specific Type 1 Regulatory T Cells from the Intestinal (application/pdf)

 
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