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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2005

Contributo in rivista

Tipo: Articolo in rivista

Titolo: MALDI-TOF mass spectrometry of a combinatorial peptide library: effect of matrix composition on signal suppression

Anno di pubblicazione: 2005

Formato: Elettronico Cartaceo

Autori: Schlosser Gitta, Pocsfalvi Gabriella, Huszar Emoke, Malorni Antonio, Hudecz Ferenc

Affiliazioni autori: Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös L. University, P. O. Box 32, Budapest 112, Hungary, H-1518 Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Science and Technology, Avellino, 83100, Italy Department of Organic Chemistry, Eötvös L. University, P. O. Box 32, Budapest 112, Hungary, H-1518

Autori CNR:

  • ANTONIO MALORNI
  • GABRIELLA KATALIN POCSFALVI

Lingua: inglese

Abstract: The effect of matrix composition on signal suppression caused by a dominant compound under MALDI ionization was studied using the combinatorial TQTXT pentapeptide library as a model system. The peptide library is composed of 19 components with all proteinogenic amino acids except cysteine in position X. From these compounds, only the Arg peptide (TQTRT) was detected with sufficient intensity in the MALDI-TOF mass spectrum under typical MALDI conditions (CCA matrix). The analysis of a set of compounds utilized as different matrix components, additives and a cationizing agent revealed that the composition of the matrix is a critical point in signal suppression. Highly improved ion yields were achieved by using a CCA/DHB mixture as a matrix. The addition of K(+) as a cationizing agent to the CCA matrix resulted in MALDI-TOF mass spectra with relative ion intensities very similar to those obtained by electrospray ionization.

Lingua abstract: inglese

Pagine da: 1590

Pagine a: 1594

Rivista:

Journal of mass spectrometry Wiley,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1076-5174

Numero volume: 19

Numero fascicolo: 12

DOI: 10.1002/jms.937

Referee: Sě: Internazionale

Indicizzato da: ISI Web of Science (WOS) [000234157300011]

Altre informazioni: 23rd Informal Meeting on Mass Spectrometry

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