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Contributo in rivista
Tipo: Articolo in rivista
Titolo: Characterization of the anti-tissue transglutaminase antibody response in nonobese diabetic mice.
Anno di pubblicazione: 2005
Autori: Sblattero D., Maurano F., Mazzarella G., Rossi M., Auricchio S., Florian F., Ziberna F., Tommasini A., Not T., Ventura A., Bradbury A., Marzari R., Troncone R.
Affiliazioni autori: *Department of Biology, University of Trieste, Trieste, Italy; +Institute of Food Science and Technology, Consiglio Nazionale delle Ricerche (CNR), Avellino, Italy; ?Department of Paediatrics and European Laboratory for the Investigation of Food Induced Diseases, University Federico II, Naples, Italy; §Department of Sciences of Reproduction and Development, University of Trieste and Istituto di Riconero e Cura a Carattere Scientifico "Burlo Garofolo", Trieste, Italy; and ¶Biosciences Division, Los Alamos National Laboratory, Los Alamos, NM Received for publication March 3, 2004. Accepted for publication February 4, 2005. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. 1 This work was financed by IRCCS Burlo Garofolo (Grant No. RF/03). 2 Address correspondence and reprint requests to Dr. Daniele Sblattero, Department of Biology, University of Trieste, Via Giorgieri 10, 34127 Trieste, Italy. E-mail address: email@example.com 3 Abbreviations used in this paper: NOD, nonobese diabetic; T1DM, type 1 diabetes mellitus; CD, celiac disease; tTG, tissue transglutaminase; scFv, single-chain Ab fragment; h tTG, human tTG; m tTG, mouse tTG; CDR, complementarity-determining region; SPL, spleen; INT, intestine.
Abstract: Type 1 diabetes mellitus is an autoimmune disorder characterized by destruction of insulin-producing pancreatic beta cells by T lymphocytes. In nonobese diabetic (NOD) mice, a role has been hypothesized for dietary gluten proteins in the onset of diabetes, and because gluten dependence is the major feature of celiac disease, together with production of Abs to the autoantigen tissue transglutaminase (tTG), we looked for the presence of anti-tTG Abs in the serum of NOD mice and, to establish their origin, analyzed the Ab repertoire of NOD mice using phage display Ab libraries. We found significant levels of serum anti-tTG Abs and were able to isolate single-chain Ab fragments to mouse tTG mainly from the Ab libraries made from intestinal lymphocytes and to a lesser extent from splenocytes. Data from NOD mice on a gluten-free diet suggest that the anti-tTG response is not gluten-dependent. The intestinal Ab response to tTG is a feature of NOD mice, but the underlying mechanisms remain obscure.
Pagine da: 5830
Pagine a: 5836
The journal of immunology
American Association of Immunologists
Numero volume: 174
Indicizzato da: ISI Web of Science (WOS) [WOS:000228630800079]
Altre informazioni: 3
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