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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2005

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Phosphorylation of p27(BBP)/eIF6 and its association with the cytoskeleton are developmentally regulated in Xenopus oogenesis.

Anno di pubblicazione: 2005

Formato: Elettronico Cartaceo

Autori: Carotenuto R., De Marco N., Biffo S., Wilding M., Vaccaro M.C., Marchisio P.C., Capriglione T., Russo G.L., Campanella C.

Affiliazioni autori: Dipartimento di Biologia Strutturale e Funzionale, Università di Napoli Federico II, Napoli (Italy), Dipartimento di Scienze dell'Ambiente e della Vita, Università del Piemonte Orientale, Alessandria (Italy) Centro per la Biologia della Riproduzione, Clinica Villa del Sole, Napoli (Italy) DIBIT e Università Vita-Salute San Raffaele Milano, (Italy) CNR, Istituto Scienze dell'Alimentazione Avellino (Italy)

Autori CNR:

  • GIAN LUIGI RUSSO

Lingua: inglese

Abstract: p27BBP/eIF6 is an evolutionarily conserved regulator of ribosomal function. It is necessary for 60S biogenesis and impedes improper joining of 40S and 60S subunits, regulated by protein kinase C or Efl1p. No data on p27BBP/eIF6 during early development of Metazoa are available. We studied the distribution, post-translational changes and association with the cytoskeleton of p27BBP/ eIF6 during Xenopus oogenesis and early development. Results indicate that p27BBP/eIF6 is present throughout oogenesis, partly associated with 60S subunits, partly free and with little cytoskeleton bound. During prophase I, p27BBP/eIF6 is detected as a single band of 27-kDa. Upon maturation induced by progesterone or protein kinase C, a serine-phosphorylated 29 kDa isoform appears and is kept throughout development to the neurula stage. Confocal microscopy showed that the distribution of p27BBP/eIF6 and its association with the cytoskeleton varies according to oogenesis stages. Briefly, in stage 6 oocytes, p27BBP/eIF6 has a limited dot-like distribution, and does not co-localize with cytokeratin, whereas upon maturation it spreads throughout the cytoplasm. After fertilization, a large fraction coalesces around cytomembranes and a cytochalasin B-sensitive co-localization with cytokeratin occurs. RNAse removes p27BBP/eIF6 from the cytokeratin fibres. Developmental data suggest a role of p27BBP/eIF6 in controlling ribosomal availability or regulating cross-talk between ribosomes and the cytoskeleton.

Lingua abstract: inglese

Pagine da: 1641

Pagine a: 1652

Rivista:

Cellular and molecular life sciences Springer Basel AG
Paese di pubblicazione: Svizzera
Lingua: inglese
ISSN: 1420-682X

Numero volume: 62

DOI: 10.1007/s00018-005-5153-9

Referee: Sì: Internazionale

Indicizzato da:

  • PubMed [15990955]
  • ISI Web of Science (WOS) [000232497100010]

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