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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2004

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Partial characterization of glycosphingolipids of Agelas sponges in their peracetylated form by liquid secondary ionization mass spectrometry and high-performance liquid chromatography combined with direct electrospray ionization mass spectrometric detectionization mass spectrometry and high-performance liquid chromatography combined with direct electrospray ionization mass spectrometric detect

Anno di pubblicazione: 2004

Formato: Elettronico

Autori: Szilagyi Z.; Pocsfalvi G.; Costantino V.; Mangoni A.; Malorni A.; Fattorusso E.

Affiliazioni autori: Department of Chemistry of Natural Products, University of Naples, Via Domenico Montesano, 49, I-80131 Naples, Italy; Proteomic and Biomolecular Mass Spectrometry Center (CESMA-ProBio), Institute of Food Science and Technology, C.N.R., via Roma, 52 a/c, I-83100 Avellino, Italy

Autori CNR:


Lingua: inglese

Abstract: Electrospray ionization (ESI) and liquid secondary ionization (LSI) mass spectrometry were applied for characterization of glycosphingolipids (GSLs) isolated in their peracetylated form from four Agelas marine sponge species. Since peracetylated glycosphingolipids are not soluble in solvents traditionally used for ESI, lithium chloride was added to the samples in order to obtain lithium cationized molecules. Although the preferred fragmentation seems to be the sequential loss of acetic acid molecules, it was found that tandem mass spectra obtained from peracetylated diglycosyl ceramides might provide direct information about the structure of the long-chain base (formation of W''/Z0 fragments). The utility of ESI and LSI in the analysis of these compounds has also been compared. It was found that the tandem mass spectra obtained by LSI-MS/MS experiments could provide information about the chain-length (carbon atom number) variations within a certain ceramide mass. Thus, from one of our samples, 25 different ceramide compositions have been identified from 8 precursor (Z0) ions. Comparison of the two ionization modes (LSI and ESI) highlights the fact that molecular mass distributions obtained by LSI-MS, especially the presence of unsaturated species, have to be interpreted carefully. For the first time a direct high-performance liquid chromatography (HPLC)/ESI-MS method was used for characterization of complex mixtures of peracetylated GSLs. The results demonstrate that HPLC/ESI-MS is able to analyze mono- and diglycosylated GSLs, and other kinds of glycolipids that are actually present in the sample. Copyright 2004 John Wiley & Sons, Ltd.

Lingua abstract: inglese

Pagine da: 2989

Pagine a: 2996


RCM. Rapid communications in mass spectrometry Heyden,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0951-4198

Numero volume: 18

Numero fascicolo: 24

DOI: 10.1002/rcm.1720

Indicizzato da: ISI Web of Science (WOS) [000225761200004]

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