Home |  English version |  Mappa |  Commenti |  Sondaggio |  Staff |  Contattaci Cerca nel sito  
Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2004

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Conjugated linoleic acid - mediated apoptosis in Jurkat T cells involves the production of reactive oxygen species.

Anno di pubblicazione: 2004

Formato: Cartaceo

Autori: Bergamo P; Luongo D; Rossi M.

Affiliazioni autori: Istituto Scienze dell'Alimentazione

Autori CNR:

  • PAOLO BERGAMO
  • DIOMIRA LUONGO
  • MAURO ROSSI

Lingua: inglese

Abstract: The pro-apoptotic ability of conjugated linoleic acid (CLA) has been partly accounted for its anticarcinogenic effect although the precise mechanism of action remains elusive. In this study we characterized the biochemical events governing CLA-mediated apoptosis in Jurkat T cells. CLA induced a time-and dose-dependent activation of caspase-3. Pre-treatment with antioxidant molecules (trolox and quercetin), antioxidant enzymes (catalase and superoxide dismutase) metal chelator (EDTA), reducing agent (N-acetyl-L-cysteine), NADPH oxidase or protein kinase C (PKC) inhibitor (diphenyleneiodinium and G 6976, respectively) suppressed CLA-mediated caspase-3 activation. Moreover, CLA treatment increased the NADPH oxidase activity and depleted the intracellular pool of reduced glutathione. These results suggested that CLA can trigger apoptosis through an oxidative stress mediated by the PKC/NADPH oxidase pathway. The proposed mechanism provides a new insight into the anticancer activity of CLA. Copyright 2004 S. Karger AG, Basel

Lingua abstract: inglese

Pagine da: 54

Pagine a: 64

Rivista:

Cellular physiology and biochemistry S. Karger
Paese di pubblicazione: Svizzera
Lingua: inglese
ISSN: 1015-8987

Numero volume: 1-2

Referee: Sė: Internazionale

Indicizzato da: PubMed [14976407]

Strutture CNR:

 
Torna indietro Richiedi modifiche Invia per email Stampa
Home Il CNR  |  I servizi News |   Eventi | Istituti |  Focus