Home |  English version |  Mappa |  Commenti |  Sondaggio |  Staff |  Contattaci Cerca nel sito  
Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2002

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Structural analysis of styrene oxide/haemoglobin adducts by mass spectrometry: identification of suitable biomarkers for human exposure evaluation

Anno di pubblicazione: 2002

Autori: Basile A., Ferranti P., Mamone G., Manco I., Pocsfalvi G., Malorni A., Acampora A., Sannolo N.

Affiliazioni autori: Adriana Basile 1 2, Pasquale Ferranti 2 3 , Gianfranco Mamone 2, Immacolata Manco 1 2, Gabriella Pocsfalvi 2, Antonio Malorni 2, Antonio Acampora 4, Nicola Sannolo 1 1Dip. di Medicina Sperimentale,Sez. Medicina del Lavoro,Igiene e Tossicologia Professionale, Seconda UniversitÓ di Napoli, 2Ist. Scienze dell'Alimentazione del C.N.R.Avellino, 3Dip. Scienza degli Alimenti, Univ. degli Studi di Napoli Federico II, 4Dip. Medicina Pubblica e Sicurezza Sociale, Univ. di Napoli Federico II

Autori CNR:

  • ANTONIO MALORNI
  • GIANFRANCO MAMONE
  • GABRIELLA KATALIN POCSFALVI

Abstract: The structural characterisation of adducts formed by the in vitro reaction of haemoglobin (Hb) with styrene oxide (SO), the most reactive metabolite of the industrial reagent styrene, was obtained by liquid chromatography/electrospray ionisation mass spectrometry (LC/ES-MS) analysis of modified tryptic peptides of human Hb chains. The reactive sites of human Hb towards SO were identified through characterisation of alkylated tryptic peptides by matrix-assisted laser desorption/ionisation with tandem mass spectrometry (MALDI-MS/MS). A procedure was set up based on this characterisation, allowing Hb modification to be assessed by monitoring SO/Hb adducts using HPLC with selected ion recording (SIR) mass spectrometry. By this methodology it was also possible to compare advantages and disadvantages of presently available strategies for the measurement of Hb adducts with SO. The results obtained could most plausibly lead to the optimisation of molecular dosimetry of SO adducts, and the analytical procedure described herein could be applied to the biological monitoring of styrene exposure in the workplace.

Pagine da: 871

Pagine a: 878

Rivista:

RCM. Rapid communications in mass spectrometry Heyden,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0951-4198

Numero volume: 16

Parole chiave:

  • LC/ES-MS
  • MALDI-MS/MS
  • haemoglobin adducts
  • styrene oxide

Strutture CNR:

 
Torna indietro Richiedi modifiche Invia per email Stampa
Home Il CNR  |  I servizi News |   Eventi | Istituti |  Focus