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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2002

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Down-regulation of ERK1 and ERK2 activity during differentiation of the intestinal cell line HT-29.

Anno di pubblicazione: 2002

Formato: Cartaceo

Autori: Luongo D; Mazzarella G; Della Ragione F; Maurano F; Rossi M.

Affiliazioni autori: Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, Avellino, Italy. Second University of Naples, Medical School, Department of Biochemistry and Biophysics F. Cedrangolo, Naples, Italy.

Autori CNR:

  • DIOMIRA LUONGO
  • FRANCESCO MAURANO
  • GIUSEPPE MAZZARELLA
  • MAURO ROSSI

Lingua: inglese

Abstract: The role and regulation of signal transduction pathways in proliferation and differentiation of intestinal epithelial cells are still poorly understood. However, growing evidences have been recently accumulated demonstrating that mitogen-activated protein kinases (MAPKs) play a pivotal function in the normal development of intestine. We have investigated, in the intestinal cell line HT-29, the regulation (namely activity and phosphorylation degree) of MAP kinases ERK 1 (p44) and ERK 2 (p42) during differentiation. Addition of fetal calf serum to HT-29 undifferentiated resting cells caused a rapid phosphorylation of both ERKs and an increase of their specific kinase activity. Moreover, nuclear translocation of ERK 1 and ERK 2 occurred concurrently to their activation, leading to the conclusion that ERK 1 and ERK 2 are classically regulated when quiescent HT-29 cells are induced to proliferate. Butyrate addition to the intestinal cell line resulted in terminal differentiation and in a selective down-regulation of ERK 2 activity (and phosphorylation degree) without any effect on ERK 1. Conversely, when HT-29 cells were differentiated by repeated passages in a glucose-free medium, we observed a progressive dephosphorylation and inactivation of p42 and p44 kinases along with the failure of serum to activate both the enzymes. Our findings suggest that, during the differentiation of intestinal cells, remarkable changes occur in ERK 1 and ERK 2 control mechanisms leading to an unresponsiveness of MAP kinase pathway.

Pagine da: 43

Pagine a: 50

Rivista:

Molecular and cellular biochemistry Nyholl
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 0300-8177

Numero volume: 231

DOI: 10.1023/A:1014476706382

Indicizzato da: ISI Web of Science (WOS) [WOS:000174186500006]

Strutture CNR:

 
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