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Contributo in rivista
Tipo: Articolo in rivista
Titolo: Keratinocyte growth factor and coeliac disease.
Anno di pubblicazione: 2001
Autori: Salvati VM, Bajaj-Elliott M, Poulsom R, Mazzarella G, Lundin KE, Nilsen EM, Troncone R, MacDonald TT
Affiliazioni autori: VM Salvati, R Troncone University Federico II, Naples; M Bajaj-Elliott, St Bartholomew’s and the Royal London School of Medicin, London, UK;R Poulsom Imperial Cancer Research Fund,London, UK;G Mazzarella ISA, CNR Avellino, Italy; K E A Lundin, E M NilsenNational Hospital,University of Oslo,Norway; T T MacDonaldUniversity of Southampton,UK
Abstract: BACKGROUND: Coeliac disease is characterised by increased epithelial renewal associated with a mucosal T cell response to gliadin. Keratinocyte growth factor (KGF) is produced by cytokine activated gut stromal cells and may be a link between mucosal T cell activation in untreated coeliac disease and epithelial hyperplasia. AIMS: To characterise expression of KGF in coeliac disease. METHODS: KGF transcripts in coeliac disease were measured by quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR) and localised using in situ hybridisation. KGF production by gluten reactive CD4+ T cell clones was examined. In addition, KGF transcripts were measured following ex vivo challenge of coeliac biopsies with a peptic-tryptic digest of gliadin. RESULTS: KGF transcripts were elevated in coeliac biopsies compared with normal controls but were not different from non-coeliac disease controls. By in situ hybridisation, KGF mRNA containing cells were present in the upper half of the lamina propria, most abundantly just under the epithelium. There was no signal from cells within the epithelium. Gluten reactive T cell clones did not make KGF. In vitro challenge of coeliac biopsies generated a strong interferon gamma response but a specific KGF response could not be detected because of an extremely high number of KGF transcripts in all cultured biopsies. CONCLUSIONS: KGF is overexpressed in coeliac biopsies and in tissues with non-coeliac enteropathy. No evidence was found for KGF production by intraepithelial lymphocytes or lamina propria T cells.
Pagine da: 176
Pagine a: 181
British Medical Association,
Numero volume: 49(2)
Indicizzato da: ISI Web of Science (WOS) [WOS:000169965000007]
Altre informazioni: Fattore d'impatto 2003: 5.883
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