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Contributo in rivista
Tipo: Articolo in rivista
Titolo: Detection of immune complexes by matrix-assisted laser desorption/ionization mass spectrometry.
Anno di pubblicazione: 2003
Autori: Schlosser G, Pocsfalvi G, Malorni A, Puerta A, de Frutos M, Vekey K.
Affiliazioni autori: Chemical Research Center, Hungarian Academy of Sciences, Budapest, Hungary Proteomic and Biomolecular Mass Spectrometry Center (CESMA-ProBio), Institute of Food Science and Technology, C.N.R., Avellino, Italy Institute of Organic Chemistry, C.S.I.C., Madrid, Spain
Abstract: Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used to detect an immune complex formed between beta-lactoglobulin and polyclonal anti-beta-lactoglobulin antibody in the gas phase. The most important experimental parameters to detect such a specific antibody-antigen complex by MALDI were the use of solutions at near-neutral pH and of sinapinic acid matrix prepared by the dried-droplet method. Under such conditions, predominantly one but also two molecules of antigen protein were complexed by the antibody. Specific formation of the antibody-antigen complex was confirmed by performing competitive reactions. Addition of antibody to a 1:1 mixture of beta-lactoglobulin and one control protein resulted not only in the appearance of the expected antibody-antigen complex, but also in a strong decrease in the free beta-lactoglobulin signal, while the abundance of the control protein was not influenced. Copyright 2003 John Wiley & Sons, Ltd.
Pagine da: 2741
Pagine a: 2747
RCM. Rapid communications in mass spectrometry
Numero volume: 17
Altre informazioni: 3
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