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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2003

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Detection of immune complexes by matrix-assisted laser desorption/ionization mass spectrometry.

Anno di pubblicazione: 2003

Autori: Schlosser G, Pocsfalvi G, Malorni A, Puerta A, de Frutos M, Vekey K.

Affiliazioni autori: Chemical Research Center, Hungarian Academy of Sciences, Budapest, Hungary Proteomic and Biomolecular Mass Spectrometry Center (CESMA-ProBio), Institute of Food Science and Technology, C.N.R., Avellino, Italy Institute of Organic Chemistry, C.S.I.C., Madrid, Spain

Autori CNR:

  • ANTONIO MALORNI
  • GABRIELLA KATALIN POCSFALVI

Abstract: Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used to detect an immune complex formed between beta-lactoglobulin and polyclonal anti-beta-lactoglobulin antibody in the gas phase. The most important experimental parameters to detect such a specific antibody-antigen complex by MALDI were the use of solutions at near-neutral pH and of sinapinic acid matrix prepared by the dried-droplet method. Under such conditions, predominantly one but also two molecules of antigen protein were complexed by the antibody. Specific formation of the antibody-antigen complex was confirmed by performing competitive reactions. Addition of antibody to a 1:1 mixture of beta-lactoglobulin and one control protein resulted not only in the appearance of the expected antibody-antigen complex, but also in a strong decrease in the free beta-lactoglobulin signal, while the abundance of the control protein was not influenced. Copyright 2003 John Wiley & Sons, Ltd.

Pagine da: 2741

Pagine a: 2747

Rivista:

RCM. Rapid communications in mass spectrometry Heyden,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0951-4198

Numero volume: 17

Altre informazioni: 3

Strutture CNR:

 
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