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Contributo in rivista
Tipo: Articolo in rivista
Titolo: Heparin-interacting sites of bovine lactoferrin are involved in anti-adenovirus activity.
Anno di pubblicazione: 2003
Autori: Di Biase AM, Pietrantoni A, Tinari A, Siciliano R, Valenti P, Antonini G, Seganti L, Superti F.
Affiliazioni autori: Laboratory of Ultrastructure, National Institute of Health, Rome, Italy Institute of Food Science and Technology, CNR, Avellino, Italy Department of Experimental Medicine, II University of Naples, Naples, Italy Department of Biology, III University of Rome, Rome, Italy Department of Public Health Sciences, University La Sapienza, Rome, Italy
Abstract: Lactoferrin, a member of the transferrin family of approximately 80 kDa, consists of a single polypeptide chain folded in two symmetric, globular lobes (N- and C-lobes), each able to bind one ferric ion. This glycoprotein, found in physiological fluids of mammals, plays an important role in immune regulation and in defense mechanisms against bacteria, fungi, parasites, and viruses. Although the antiviral activity of lactoferrin is one of the major biological functions of such protein, the mechanism of action is still under debate. We have investigated both the role of tryptic fragments of bovine lactoferrin and the mechanism of lactoferrin antiviral effect toward adenovirus infection in HEp-2 cells. The results obtained demonstrated that the anti-adenovirus activity of lactoferrin is mediated by the N-terminal half of the protein as the N-lobe was able to inhibit adenovirus infection, even if at lower extent than undigested lactoferrin, whereas C-lobe was ineffective. The results also showed that the anti-adenovirus action of lactoferrin and of its N-terminal peptide lactoferricin took place on virus attachment to cell membrane, mainly through competition for common glycosaminoglycan receptors. The data provide evidence that the anti-adenovirus activity of lactoferrin is mediated mainly by the cluster of positive charges at the N-terminus of whole molecule and that the N-terminal peptide lactoferricin alone is sufficient to prevent infection.
Pagine da: 495
Pagine a: 502
Journal of medical virology
Alan R. Liss,
Numero volume: 69(4)
Altre informazioni: 20
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