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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2001

Contributo in rivista

Tipo: Articolo in rivista

Titolo: A novel approach for identification and measurement of hemoglobin adducts with 1,2,3,4-diepoxybutane by liquid chromatography/electrospray ionisation mass spectrometry and matrix-assisted laser desorption/ionisation tandem mass spectrometry.

Anno di pubblicazione: 2001

Autori: Basile A, Ferranti P, Pocsfalvi G, Mamone G, Miraglia N, Caira S, Ambrosi L, Soleo L, Sannolo N, Malorni A.

Affiliazioni autori: Adriana Basile 1 2, Pasquale Ferranti 1 3, Gabriella Pocsfalvi1,Gianfranco Mamone 1, Nadia Miraglia 1, Simonetta Caira 1 3,Luigi Ambrosi 4, Leonardo Soleo 2, Nicola Sannolo 1 5, Antonio Malorni 1 1Centro Intern. Servizi di Spettrom. di Massa,ISA- C.N.R.Avellino 2Dip. Medicina Interna e Medicina Pubblica,Uni. degli Studi di Bari 3Dip. Scienza degli Alimenti, Univ. Studi di Napoli Federico II 4FacoltÓ Medicina e Chirurgia,Univ.Studi di Foggia 5Ist. Medicina del Lavoro, Seconda Univ.di Napoli

Autori CNR:

  • SIMONETTA CAIRA
  • ANTONIO MALORNI
  • GIANFRANCO MAMONE
  • GABRIELLA KATALIN POCSFALVI

Abstract: The structural characterisation of the adducts formed by in vitro interaction of hemoglobin (Hb) with 1,2,3,4-diepoxybutane (DEB), the most reactive 1,3-butadiene (BD) metabolite, was obtained by liquid chromatography/electrospray ionisation mass spectrometry (LC/ES-MS) analysis of modified tryptic peptides of human hemoglobin chains. The reactive sites of human hemoglobin towards DEB and its hydroxylated derivatives (trihydroxybutyl (THB)-derivatives) were identified through the characterisation of alkylated tryptic peptides by matrix-assisted laser desorption/ionisation tandem mass spectrometry (MALDI-MS/MS). Based on this characterisation, a procedure was set up to measure the Hb-adducts of THB-derivatives by isotope dilution mass spectrometry with the use of a deuterated peptide standard. The results obtained here could permit optimisation of molecular dosimetry of BD-adducts, and extension of the analysis to the biological monitoring of occupational exposure to butadiene.

Pagine da: 527

Pagine a: 540

Rivista:

RCM. Rapid communications in mass spectrometry Heyden,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0951-4198

Numero volume: 15(8)

Parole chiave:

  • LC/ES-MS
  • MALDI-MS/MS
  • Hemoglobin
  • 1,2,3,4-diepoxybutane
  • 1,3-butadiene

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