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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2001

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Mass spectrometric identification of a candidate biomarker peptide from the in vitro interaction of epichlorohydrin with red blood cells.

Anno di pubblicazione: 2001

Formato: Cartaceo

Autori: Miraglia N, Pocsfalvi G, Ferranti P, Basile A, Sannolo N, Acampora A, Soleo L, Palmieri F, Caira S, De Giulio B, Malorni A.

Affiliazioni autori: N. Miraglia1 2 3,G. Pocsfalvi1,P. Ferranti1 4,A. Basile1 5,N. Sannolo1 2,A. Acampora1 3,L. Soleo 5,F. Palmieri6,S. Caira4,B. De Giulio1,A. Malorni1 1C. Intern. Servizi di Spettrom. di Massa ISA-CNR Avellino, 2Dip. Med. del Lavoro, II Univ. Studi di Napoli, 3Dip. Med. Pubblica e Sicur. Soc., Univ. di Napoli Federico IIĄ¯, 4Dip. Scienza degli Alim, Univ. di Napoli Federico IIĄ¯, 5Dip. Med. Interna e Med. Pubblica, Universit¨¤ di Bari, 6Dip. Farmaco-Biologico, Univ. degli Studi di Bari

Autori CNR:


Lingua: inglese

Abstract: The reaction products of epichlorohydrin with human alpha- and beta- globins, obtained through in vitro incubation of these compounds and red blood cells, were determined by using reversed-phase high-performance liquid chromatography (RP-HPLC), electrospray ionization mass spectrometry and matrix-assisted laser desorption/ionization tandem mass spectrometry. The alpha-globin was much more reactive than the beta-globin. At low incubation ratios, approximating the order of magnitude of epichlorohydrin concentration as found in workplaces, the only modified peptide still detectable was the 62-90 belonging to the alpha-chain and carrying an incremental mass of 92 u on either His72 or His89. Given that the two peptides co-eluted in a single chromatographic peak during RP-HPLC separation, they could be chosen as suitable biomarkers for quantification in the setting up of a new methodology for the biological monitoring of persons occupationally exposed, replacing currently known procedures. Copyright 2001 John Wiley & Sons, Ltd. Abbreviations: ECH epichlorohydrin Hb hemoglobin GC/MS gas chromatography/mass spectrometry ESMS electrospray mass spectrometry MALDI-MS matrix-assisted laser desorption/ionization mass spectrometry MS/MS tandem mass spectrometry LC liquid chromatography RP-HPLC reversed-phase high-performance liquid chromatography TFA trifluoroacetic acid SIM selected ion monitoring u, mass unit. The standard one-or three-letter code has been used for the amino acids.

Lingua abstract: inglese

Pagine da: 47

Pagine a: 57


Journal of mass spectrometry Wiley,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1076-5174

Numero volume: 36(1)

DOI: 10.1002/jms.103

Referee: Sė: Internazionale

Indicizzato da:

  • ISI Web of Science (WOS) [WOS:000166949600007]
  • PubMed [11180646]

Parole chiave:

  • epichlorohydrin
  • hemoglobin adduct
  • mass spectrometry
  • occupational exposure
  • biomonitoring applications

Strutture CNR:


Allegati: Mass spectrometric identification of a candidate biomarker peptide from the in vitro interaction of epichlorohydrin with red blood cells. (application/pdf)

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