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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2003

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Casein phosphoproteome: identification of phosphoproteins by combined mass spectrometry and two-dimensional gel electrophoresis

Anno di pubblicazione: 2003

Autori: Mamone, G., Caira, S., Garro, G., Nicolai, A., Ferranti, P., Picariello, G., Malorni, A., Chianese, L., Addeo, F.

Affiliazioni autori: Gianfranco Mamone,Simonetta Caira,Gianluca Picariello,Antonio Malorni: Istituto di Scienze dell’Alimentazione del C.N.R.,Avellino, Italy. Lina Chianese,Adalgisa Nicolai:Dipartimento di Scienza degli Alimenti Università degli Studi di Napoli “Federico II”,Portici, Italy. Giuseppina Garro,Pasquale Ferranti,Francesco Addeo:Istituto di Scienze dell’Alimentazione del C.N.R.,Avellino, Italy e Dipartimento di Scienza degli Alimenti Università degli Studi di Napoli “Federico II”,Portici, Italy

Autori CNR:

  • SIMONETTA CAIRA
  • ANTONIO MALORNI
  • GIANFRANCO MAMONE
  • GIANLUCA PICARIELLO

Abstract: We report a fast and easy-to-use procedure that combines polyacrylamide gel electrophoresis with matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF) and nanoelectrospray-tandem mass spectrometry (nES-MS/MS) analysis for the identification of casein components and defined phosphorylated sites. This methodology ensured identification of more than 30 phosphorylated proteins, five beta-, fifteen alpha(s1)-, ten alpha(s2)-, and four kappa-casein (CN) components, including nonallelic, differently phosphorylated, and glycosylated forms. The sugar motif covalently bound to kappa-CN was identified as chains, trisaccharide GalNAc, Gal, NeuGc, and tetrasaccharide 1GalNAc, 1Gal, 2NeuGc. Also identified was a biantennary chain made up of both chains of trisaccharide 1GalNAc, 1Gal, 1NeuGc, and tetrasaccharide 1GalNAc, 1Gal, 2NeuGc moiety on a single kappa-CN component. The phosphate group on site Ser12 of tryptic peptide 8-22 of most phosphorylated alpha(s1)-CN (11 phosphate groups) was localized and the oligosaccharide sequence of the main tryptic glycopeptides of two kappa-CN components was determined by means of MS/MS analysis.

Pagine da: 2824

Pagine a: 2837

Rivista:

Electrophoresis Wiley-VCH.
Paese di pubblicazione: Germania
Lingua: inglese
ISSN: 0173-0835

Numero volume: 24

Parole chiave:

  • Proteomics
  • Casein phosphoproteome
  • Glycosylation
  • Mass spectrometry
  • Twodimensional

Altre informazioni: 8

Strutture CNR:

 
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