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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2003

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Molecular recognition between Azotobacter vinelandii rhodanese and a sulfur acceptor protein

Anno di pubblicazione: 2003

Autori: Cereda, A., Forlani, F., Iametti, S., Bernhardt, R., Ferranti, P., Picariello, G., Pagani, S., Bonomi, F.

Autori CNR:


Abstract: The occurrence of rhodanese-like proteins in the major evolutionary phyla, together with the observed abundance of these proteins also within the same genome, suggests that their function cannot be limited to cyanide scavenging. The aim of the present study was to investigate whether Azotobacter vinelandii RhdA, an enzyme possessing unique biochemical and structural features with respect to other members of rhodanese homology superfamily, could recognize a suitable protein as a potential acceptor of the sulfane sulfur held on its catalytic Cys residue. Both the potential sulfur-delivery RhdA-S and the sulfur-deprived RhdA were found to interact with either holo- or apo-adrenodoxin, the 'substrate' protein used in this work. Interaction of RhdA-S with apo-adrenodoxin led to mobilization of RhdA-S sulfane sulfur. Under appropriate conditions, the sulfur released from RhdA-S was productively used for 2Fe-2S cluster reconstitution to yield holo-adrenodoxin from apo-adrenodoxin in the absence of any other sulfur source. A comparison of the reactivity of RhdA-S with protein and non-protein thiols allowed also some insights into the accessibility of the sulfane sulfur carried by RhdA.

Pagine da: 1473

Pagine a: 1481


Biological chemistry de Gruyter.
Paese di pubblicazione: Germania
Lingua: inglese
ISSN: 1431-6730

Numero volume: 384

Altre informazioni: 3

Strutture CNR:

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