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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2003

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Effects of conjugated linoleic acid on growth and cytokine expression in Jurkat T cells.

Anno di pubblicazione: 2003

Formato: Cartaceo

Autori: Luongo D, Bergamo P, Rossi M.

Affiliazioni autori: Istituto di scienza dell'alimentazione

Autori CNR:

  • PAOLO BERGAMO
  • DIOMIRA LUONGO
  • MAURO ROSSI

Lingua: inglese

Abstract: Conjugated linoleic acid (CLA) has shown beneficial properties in animal models including anti-cancer, anti-atherogenic and anti-diabetic effects, while contrasting immunological effects were reported. While its anti-inflammatory activity has been associated to inhibition of arachidonic acid biosynthesis and to peroxisome proliferator-activated receptors (PPARs) activity, the molecular pathways underlying its immunoenhancing activity are essentially unknown. The aim of our study was to examine whether CLA showed specific effects in vitro on a T cell model, represented by the Jurkat cell line. CLA was found non toxic for Jurkat in the range 50-200 microM, as assessed by LDH release; however, incubation with 50 microM CLA was associated to a significant inhibitory effect on cell proliferation. The analysis of IL-2 and IFN-gamma transcript levels, produced in stimulated Jurkat cells, showed an increased expression of both cytokines in CLA-treated cells. Interestingly, the increased induction of IL-2 but not of IFN-gamma mRNA, could be suppressed by co-incubation with Go 6976, a protein kinase C (PKC) inhibitor. Co-incubation with superoxide dismutase (SOD) or N-acetyl-L-cysteine (NAC) restored the basal levels of RNA synthesis for both cytokines. Taken together, these results suggest a specific role for dietary CLA in the modulation of the immune response in a T cell line model that is mediated, at least in part, by PKC and through the production of oxidative molecules.

Pagine da: 195

Pagine a: 201

Rivista:

Immunology letters Elsevier
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 0165-2478

Numero volume: 90

Altre informazioni: 15

Strutture CNR:

 
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