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Contributo in rivista
Tipo: Articolo in rivista
Titolo: Celiac disease association with CD8+ T cell responses: identification of a novel gliadin-derived HLA-A2-restricted epitope.
Anno di pubblicazione: 2003
Formato: Elettronico Cartaceo
Autori: Gianfrani C, Troncone R, Mugione P, Cosentini E, De Pascale M, Faruolo C, Senger S, Terrazzano G, Southwood S, Auricchio S, Sette A.
Affiliazioni autori: Institute of Food Science and Technology, Consiglio Nazionale delle Ricerche, Avellino, Italy EPIMMUNE, San Diego, CA, USA Department of Pediatrics, University of Naples, naples, Italy
Abstract: One of the diagnostic hallmarks of the histological lesions associated with celiac disease is the extensive infiltration of the small intestinal epithelium by CD8(+) T cells of unknown Ag specificity. In this study, we report recognition of the gliadin-derived peptide (A-gliadin 123-132) by CD8(+) T lymphocytes from celiac patients. A-gliadin 123-132-specific IFN-gamma production and cytotoxic activity were detected in PBMCs derived from patients on gluten-free diet, but not from either celiac patients on gluten-containing diet or healthy controls. In contrast, A-gliadin 123-132-specific cells were isolated from small intestine biopsies of patients on either gluten-free or gluten-containing diets. Short-term T cell lines derived from the small intestinal mucosa and specific for the 123-132 epitope recognized human APC pulsed with either whole recombinant alpha-gliadin or a partial pepsin-trypsin gliadin digest. Finally, we speculate on a possible mechanism leading to processing and presentation of class I-restricted gliadin-derived epitopes in celiac disease patients.
Pagine da: 2719
Pagine a: 2726
The Journal of immunology
Williams & Wilkins,
Numero volume: 170
Referee: Sė: Internazionale
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