Torna all'elenco Contributi in rivista anno 2002

Tipo: Articolo in rivista

Titolo: Anti-tissue transglutaminase antibodies from coeliac patients inhibit transglutaminase activity both in vitro and in situ.

Anno di pubblicazione: 2002

Autori: Esposito C, Paparo F, Caputo I, Rossi M, Maglio M, Sblattero D, Not T, Porta R, Auricchio S, Marzari R, Troncone R.

Affiliazioni autori: Department of Chemistry, University of Salerno, Baronissi, Italy.Department of Pediatrics, University of Naples, Naples, Italy Istituto di Scienze dell'Alimentazione, CNR, Avellino, Italy

Autori CNR:

• MAURO ROSSI

Abstract: BACKGROUND AND AIMS: Coeliac disease (CD) is a multifactorial disorder which has an autoimmune component characterised by the occurrence of disease specific autoreactive antibodies against the enzyme tissue transglutaminase (tTG). The aim of this study was to investigate whether binding of antibodies to the enzyme influences tTG activity. METHODS: tTG activity was assayed in the presence of immunoglobulin A (IgA) and immunoglobulin G (IgG) purified from the serum of coeliac patients, CUB 7402 (an anti-tTG mouse monoclonal antibody), and human anti-tTG monoclonal antibodies derived from both intestinal lymphocytes from three patients with CD and from peripheral blood lymphocytes from healthy subjects. For our studies we used calcium treated and untreated recombinant human tTG. Furthermore, the effects of antibodies were determined by immunohistochemical detection of tTG activity in sections of human umbilical cord. RESULTS: IgG and IgA from CD patients inhibited tTG activity in vitro in a dose dependent manner, with a different rate of inhibition among patients. The monoclonal antibody CUB 7402 and human monoclonal antibodies displayed a dose dependent inhibitory effect towards the catalytic activity of the enzyme, both in vitro and in situ. Preincubation of tTG with CaCl(2) caused loss of the inhibitory effect due to CUB 7402 but not that caused by human monoclonal antibodies. CONCLUSIONS: Purified CD IgA, IgG, as well as human anti-tTG monoclonal antibodies inhibited the enzymatic activity of human tTG both in vitro and in situ.

Pagine da: 177

Pagine a: 181

Rivista:

Gut British Medical Association,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0017-5749

Numero volume: 51

Parole chiave:

• celiachia
• transglutaminasi
• autoimmunità

Altre informazioni: Fattore d'impatto 2003: 5.883

Strutture CNR:

• Istituto di scienza dell'alimentazione (ISA)