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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2003

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Toxicity of melanin-free ink of Sepia officinalis to transformed cell lines: identification of the active factor as tyrosinase

Anno di pubblicazione: 2003

Formato: Elettronico Cartaceo

Autori: Russo GL, De Nisco E, Fiore G, Di Donato P, d'Ischia M, Palumbo A

Affiliazioni autori: Institute of Food Science, National Research Council, Avellino, Italy Zoological Station "Anton Dohrn", Naples, Italy Department of Organic Chemistry and Biochemistry, University of Naples "Federico II", Naples, Italy

Autori CNR:

  • GIAN LUIGI RUSSO

Lingua: inglese

Abstract: The melanin-free ink of the cephalopod Sepia officinalis is shown to contain a heat labile proteinaceous component toxic to a variety of cell lines, including PC12 cells. Gel filtration chromatography indicated that the toxic component was concentrated in those fractions eluted at a molecular weight higher than 100 kDa and exhibiting the highest tyrosinase activity. SDS-PAGE analysis of the active fractions displayed a single major band migrating at an approximate molecular weight of 100 kDa, identical with that of the single tyrosinase band in the melanin-free ink. These data unambiguously demonstrated the identity of the toxic component with tyrosinase. Treatment of purified Sepia as well as of mushroom tyrosinase with an immobilized version of proteinase K resulted in a parallel loss of tyrosinase activity and cytotoxicity. Sepia apotyrosinase was ineffective in inducing cytotoxicity in PC12 cells. Purified Sepia tyrosinase was found to induce a significant increase in caspase 3 activity in PC12 cells, leading eventually to an irreversible apoptotic process. Overall, these results disclose a hitherto unrecognized property of tyrosinase that may lead to a reappraisal of its biological significance beyond that of a mere pigment producing enzyme.

Lingua abstract: inglese

Pagine da: 293

Pagine a: 299

Rivista:

Biochemical and biophysical research communications Elsevier [etc.]
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0006-291X

Numero volume: 308

Numero fascicolo: 2

Referee: Sė: Internazionale

Indicizzato da: PubMed [12901867]

Strutture CNR:

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