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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2020

Contributo in rivista

Tipo: Articolo in rivista

Titolo: A carotenoid-enriched extract from pumpkin delays cell proliferation in a human chronic lymphocytic leukemia cell line through the modulation of autophagic flux

Anno di pubblicazione: 2020

Formato: Elettronico

Autori: Stefania Moccia, Maria Russo, Miriana Durante, Marcello S. Lenucci, Giovanni Mita, Gian Luigi Russo

Affiliazioni autori: National Research Council, Institute of Food Sciences, 83100, Avellino Institute of Sciences of Food Production Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100, Lecce, Italy

Autori CNR:

  • MIRIANA DURANTE
  • GIOVANNI MITA
  • STEFANIA MOCCIA
  • GIAN LUIGI RUSSO
  • MARIA RUSSO

Lingua: inglese

Abstract: Chronic lymphocytic leukemia (CLL) is the most frequent form of leukemia in adult population. From an asymptomatic state, which can lay for years, CLL rapidly evolves becoming fatal. Apoptosis resistance and induction of a protective form of autophagy are possible explanations of the poor responsivity of CLL to conventional and innovative therapeutic drugs. Carotenoids, including ?- and ?-carotene, lycopene, and derivatives, such as retinoic acid, have been studied for their significant antiproliferative and differentiating activity in cancer. Here, we report that a carotenoid-enriched extract (CE) obtained from pumpkin by supercritical CO2 extraction showed an anti-proliferative effect on HG3 cell line derived from human B-CLL cells. CE induced a 40% delay in cell proliferation compared to untreated cells, without signs of cytotoxicity. This delay was associated with p27Kip1 over-expression, AMPK activation and modulation of autophagy flux. In HG3 cells treated with CE, we detected a 30% autophagosome intracellular increase and changes in the expression of canonical biochemical markers of autophagy (LC3-II, p62). These results suggest the presence in the CE of a pool of bioactive carotenoids acting additively or synergistically in retarding cancer cell growth.

Lingua abstract: inglese

Pagine da: 74

Pagine a: 82

Pagine totali: 9

Rivista:

Current trends in biotechnology and chemical research Maharishi Markandeshwar University
Paese di pubblicazione: India
Lingua: inglese
ISSN: 2249-4073

DOI: 10.1016/j.crbiot.2020.05.001

Referee: Ś: Internazionale

Stato della pubblicazione: Published version

Parole chiave:

  • Chronic lymphocytic leukemiaAutophagyCarotenoidsHG3 cell line

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