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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2020

Contributo in rivista

Tipo: Articolo in rivista

Titolo: In Celiac Disease Patients the In Vivo Challenge with the Diploid Triticum monococcum Elicits a Reduced Immune Response Compared to Hexaploid Wheat

Anno di pubblicazione: 2020

Formato: Elettronico

Autori: Picascia S, Camarca A, Malamisura M, Mandile R, Galatola M, Cielo D, Gazza L, Mamone G, Auricchio S, Troncone R, Greco L, Auricchio R, Gianfrani C

Affiliazioni autori: Institute of Biochemistry and Cell Biology, National Research Council, Naples, Italy. Institute of Food Sciences, National Research Council, Avellino, Italy. Department of Medical Translational Sciences, Section of Pediatrics, University of Naples, Federico II, Italy. CREA Research Centre for Engineering and Agro-Food Processing, Rome, Italy. European Laboratory for the Food Induced Diseases (E.L.F.I.D), University of Naples, Federico II, Italy. Abstract

Autori CNR:

  • ALESSANDRA CAMARCA
  • CARMELA GIANFRANI
  • GIANFRANCO MAMONE
  • STEFANIA PICASCIA

Lingua: inglese

Abstract: SCOPE: Gluten from the diploid wheat Triticum monococcum (TM) has low content of immunostimulatory sequences and a high gastro-intestinal digestibility. We analysed gluten-reactive T cells elicited by diploid and hexaploid (Triticum aestivum-TA) wheat in celiac disease (CD) patients upon a brief oral challenge. METHODS AND RESULTS: Seventeen patients with CD (median age 13 years) consumed for 3-days sandwiches made with TM (cultivar Norberto-ID331, N = 11), or TA (cultivar Sagittario, N = 11) flours, corresponding to 12 gr of gluten/die. Immunostimulatory properties were assessed in blood by measuring the IFN-?-secreting T cells by EliSpot and the expression of inflammatory cytokines/receptors (IL-12A, IL-15, IL-18RAP, IFN-?) by qPCR. TA mobilized a remarkable number of gliadin specific, IFN-?-secreting T cells (p < 0.05), whilst no significant cell mobilization was induced by TM (p = ns). Similar results were obtained in response to five immunogenic peptides from ?-, ?-, and ?-gliadins, although with a large individual variability. An increased mRNA expression for IL-12A and IFN-? was detected in group eating TA compared to those consuming TM (p < 0.05). CONCLUSIONS: Although Triticum monococcum is a cereal not suitable for the diet of celiacs, we demonstrated that this diploid wheat elicits a reduced in vivo T-cell response compared to Triticum aestivum in celiac patients. This article is protected by copyright. All rights reserved.

Lingua abstract: inglese

Rivista:

Molecular nutrition & food research Wiley-VCH-Verl..
Paese di pubblicazione: Germania
Lingua: inglese
ISSN: 1613-4125

DOI: 10.1002/mnfr.201901032

Referee: Sė: Internazionale

Stato della pubblicazione: Published version

Parole chiave:

  • celiac disease
  • triticum monococcum
  • brief gluten oral challenge

Strutture CNR:

 
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