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Contributo in rivista
Tipo: Articolo in rivista
Titolo: The murine enterocyte cell line Mode-K is a novel and reliable in vitro model for studies on gluten toxicity
Anno di pubblicazione: 2020
Autori: Luongo D.; Treppiccione L.; Maurano F.; Rossi M.; Bergamo P.
Affiliazioni autori: Institute of Food Sciences, National Research Council (CNR-ISA), Avellino, Institute of Food Sciences, National Research Council (CNR-ISA), Avellino, Italy, , , Italy; Institute of Food Sciences, National Research Council (CNR-ISA), Avellino, Institute of Food Sciences, National Research Council (CNR-ISA), Avellino, Italy, , , Italy
Abstract: The resemblance of physiological traits of cell lines with their target/original tissue is an important prerequisite for the choice of the in vitro model. Although cytoprotective defenses, activated by the nuclear factor erythroid 2-related factor2 (Nrf2), have a preeminent importance in intestinal protection, nevertheless their levels in in vitro models have been never compared with those of their original tissue. Basal level of Nrf2-mediated defenses in murine enterocyte cells (Mode-K) and in human colon adenocarcinoma cells -at differentiated (DCaco2) or confluent stage (CCaco2)- were compared with those found in mouse or human duodenum. The pro-oxidant and cytotoxic effects of peptic-tryptic digest of gluten prepared from wheat bread (PT-b), einkorn (PT-e) or durum wheat (PT-d) were evaluated in Mode-k and DCaco2 by combining enzymatic, immune-enzymatic and real-time PCR assay. The results presented reveal that Mode-k cells resemble cytoprotective defenses of human/murine duodenum and are more susceptible to pro-oxidant, cytotoxic and pro-inflammatory effect of gliadin digest (in comparison with Caco2). Prolamins digests from the considered wheat exhibit different inhibitory effect on Nrf2-mediated defenses (PT-b > PT-e > PT-d). These data indicate, for the first time, that Mode-k are a reliable model for investigating wheat prolamins toxicity and for evaluating the signaling pathway of gluten-associated disease.
Lingua abstract: inglese
Food and chemical toxicology
Numero volume: 140
Referee: Sė: Internazionale
Stato della pubblicazione: Preprint
Indicizzato da: Scopus [2-s2.0-85083225037]
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