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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2020

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Development of support based on chitosan and cellulose nanocrystals for the immobilization of anti-Shiga toxin 2B antibody

Anno di pubblicazione: 2020

Autori: Shankar, Shiv; Baraketi, Amina; D'Auria, Sabato; Fraschini, Carole; Salmieri, Stephane; Jamshidian, Majid; Etty, Marie Christine; Lacroix, Monique

Affiliazioni autori: Canadian Irradiat Ctr; CNR; FPInnovations

Autori CNR:

  • SABATO D'AURIA

Lingua: inglese

Abstract: This work describes the development of membrane based on chitosan (CHI), cellulose nanocrystals (CNCs), and glycerol (GLY), and optimization of the formulation for immobilization of monoclonal anti-Shiga toxin 2B antibody (mAnti-stx2B-Ab) for E. coli O157:H7 detection. The effect of CHI deacetylation degree & viscosity, CNCs and GLY concentrations on Anti-stx2B-Ab immobilization efficiency was evaluated. Fractional factorial and Box-Behnken designs were applied to screen the effects of compounds interactions and optimize their concentrations for detection of Anti-stx2B-Ab. The results demonstrated that the use of 0.6 % (w/v) CNCs improved significantly the Anti-stx2B-Ab immobilization and the level of signal detection. The detection limit of Escherichia coli O157:H7 by developed optimized membrane is 1 log CFU/mL. The time needed for detection of E. coli O157:H7 was only 4 h of enrichment compared to 24 h with conventional methods. The developed immobilization support has potential for future pathogen detection in food and biomedical samples.

Lingua abstract: inglese

Pagine totali: 8

Rivista:

Carbohydrate polymers Applied Science Publishers.
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0144-8617

Numero volume: 232

DOI: 10.1016/j.carbpol.2019.115785

Stato della pubblicazione: Published version

Indicizzato da: ISI Web of Science (WOS) [000507231800016]

Parole chiave:

  • Cellulose nanocrystals
  • Chitosan
  • Antibody immobilization
  • Fractional factorial design
  • Box-Behnken design

Strutture CNR:

 
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