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Contributo in rivista
Tipo: Articolo in rivista
Titolo: Development of support based on chitosan and cellulose nanocrystals for the immobilization of anti-Shiga toxin 2B antibody
Anno di pubblicazione: 2020
Autori: Shankar, Shiv; Baraketi, Amina; D'Auria, Sabato; Fraschini, Carole; Salmieri, Stephane; Jamshidian, Majid; Etty, Marie Christine; Lacroix, Monique
Affiliazioni autori: Canadian Irradiat Ctr; CNR; FPInnovations
Abstract: This work describes the development of membrane based on chitosan (CHI), cellulose nanocrystals (CNCs), and glycerol (GLY), and optimization of the formulation for immobilization of monoclonal anti-Shiga toxin 2B antibody (mAnti-stx2B-Ab) for E. coli O157:H7 detection. The effect of CHI deacetylation degree & viscosity, CNCs and GLY concentrations on Anti-stx2B-Ab immobilization efficiency was evaluated. Fractional factorial and Box-Behnken designs were applied to screen the effects of compounds interactions and optimize their concentrations for detection of Anti-stx2B-Ab. The results demonstrated that the use of 0.6 % (w/v) CNCs improved significantly the Anti-stx2B-Ab immobilization and the level of signal detection. The detection limit of Escherichia coli O157:H7 by developed optimized membrane is 1 log CFU/mL. The time needed for detection of E. coli O157:H7 was only 4 h of enrichment compared to 24 h with conventional methods. The developed immobilization support has potential for future pathogen detection in food and biomedical samples.
Lingua abstract: inglese
Pagine totali: 8
Applied Science Publishers.
Numero volume: 232
Stato della pubblicazione: Published version
Indicizzato da: ISI Web of Science (WOS) 
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