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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2020

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Fluorescence polarization assay to detect the presence of traces of ciprofloxacin

Anno di pubblicazione: 2020

Autori: El Kojok, Hiyam; El Darra, Nada; Khalil, Mahmoud; Capo, Alessandro; Pennacchio, Angela; Staiano, Maria; Camarca, Alessandra; D'Auria, Sabato; Varriale, Antonio

Affiliazioni autori: Beirut Arab Univ; Beirut Arab Univ; CNR

Autori CNR:

  • ALESSANDRA CAMARCA
  • ALESSANDRO CAPO
  • SABATO D'AURIA
  • ANGELA PENNACCHIO
  • MARIA STAIANO
  • ANTONIO VARRIALE

Lingua: inglese

Abstract: Detection of ciprofloxacin residues in milk by sensitive and rapid methods is of great interest due to its use in the treatment of dairy livestock health. Current analytical approaches to antibiotics detection, are laboratory-based methods and they are time-consuming and require trained personnel. To cope this problem, we propose an assay, based on fluorescence polarization principle, able to detect the presence of ciprofloxacin in diluted milk sample without any pre-treatment. The proposed method is based on the use of ciprofloxacin-protein conjugate labeled with near infrared fluorescence dye, which upon binding to specific antibody causes an increase of the fluorescence polarization emission signal. The developed assay allows for the detection of ciprofloxacin at a concentration of 1ppb, which represents an amount lower than the maximum residual limit (MRL) of ciprofloxacin in milk, as set by the European Union regulation (100 ppb).

Lingua abstract: inglese

Pagine totali: 6

Rivista:

Scientific reports Nature Publishing Group
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 2045-2322

Numero volume: 10

Numero fascicolo: 1

DOI: 10.1038/s41598-020-61395-3

Stato della pubblicazione: Published version

Indicizzato da: ISI Web of Science (WOS) [000520964500002]

Parole chiave:

  • Biosensors

Strutture CNR:

 
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