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Contributo in rivista
Tipo: Articolo in rivista
Titolo: IBD: Role of intestinal compartments in the mucosal immune response
Anno di pubblicazione: 2020
Formato: Elettronico Cartaceo
Autori: Iacomino G.; Rotondi Aufiero V.; Iannaccone N.; Melina R.; Giardullo N.; De Chiara G.; Venezia A.; Taccone F.S.; Iaquinto G.; Mazzarella G.
Affiliazioni autori: Institute of Food Sciences-CNR, Avellino, Institute of Food Sciences-CNR, Avellino, Italy, , Italy; Department of Gastroenterology, San G. Moscati Hospital, Avellino, Department of Gastroenterology, San G. Moscati Hospital, Avellino, Italy, , Italy; Department of AnatomicPathology, San G. Moscati Hospital, Avellino, Department of AnatomicPathology, San G. Moscati Hospital, Avellino, Italy, , Italy; Department of Intensive Care, Erasme Hospital, Universit e Libre de Bruxelles, Brussels, Department of Intensive Care, Erasme Hospital, Universit e Libre de Bruxelles, Brussels, Belgium, , Belgium; Division of Gastroenterology, Santa Rita Hospital, Atripalda, Av, Division of Gastroenterology, Santa Rita Hospital, Atripalda, Av, Italy, , Italy
Abstract: Background and aims: Laser capture microdissection (LCM) is a powerful tool for the isolation of specific tissue compartments. We aimed to investigate the mucosal immune response that takes place in different intestinal compartments of IBD patients, dissected by LCM, analyzing cytokines expression profile and endoplasmic reticulum (ER) stress markers. Methods: Frozen sections of gut were obtained from patients with Crohn's disease (CD), ulcerative colitis (UC) and from controls. Using LCM, surface epithelium (SE) and lamina propria (LP) compartments were isolated and total RNA extracted. The relative expression of Th1, Th17 and Treg cytokines was evaluated by quantitative reverse transcriptase real-time PCR (qRT-PCR), in addition to the assessment of mRNA splicing of the transcription factor X-box binding protein-1 (XBP1). Human neutrophil elastase (HNE) and the transcription factor forkhead box P3 (Foxp3) were also analyzed by immunohistochemistry. Results: The increased expression of IL-17 was observed in both intestinal compartments of IBD patients when compared to controls. IFN- ?, TNF-?, IL-10, HNE and Foxp3 were overexpressed in the LP compartment of both IBD patients as compared to controls. An upregulation of IFN-? and an infiltration of HNE+ cells was found in the SE of patients with UC. Splicing of XBP1 mRNA was recognized in both intestinal compartments of IBD patients when compared to controls. Conclusions: In IBD patients, both intestinal compartments are involved in Th17 response, whereas, LP compartment plays a prominent role in Th1 and Treg immune responses. Nevertheless, high level of IFN- ? was found in the SE of UC patients, suggesting that this compartment is involved in the Th1 immune response. Our data also suggested that ER stress signalling is active in both LP and SE compartment of IBD patients, thus advocating that ER stress and immunity are intertwined.
Lingua abstract: inglese
Urban u. Fischer.
Referee: Sė: Internazionale
Stato della pubblicazione: Published version
Indicizzato da: Scopus [2-s2.0-85072511572]
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