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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2018

Contributo in rivista

Tipo: Articolo in rivista

Titolo: In vitro intestinal epithelium responses to titanium dioxide nanoparticles

Anno di pubblicazione: 2018

Formato: Elettronico Cartaceo

Autori: Paola Pedata1,, Giulia Ricci1,, Livia Malorni2, Antonella Venezia2, Marcella Cammarota1, Maria Grazia Volpe2, Nunzia Iannaccone2, Vincenzo Guida1, Chiara Schirardi1, Marco Romano3, Giuseppe Iacomino2

Affiliazioni autori: 1) Dipartimento di Medicina Sperimentale, Università degli Studi della Campania Luigi Vanvitelli, Napoli, Italy. 2) Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, Avellino, Italy. 3) Dipartimento di Internistica Clinica e Sperimentale "F. Magrassi", Università degli Studi della Campania Luigi Vanvitelli, Napoli, Italy.

Autori CNR:

  • GIUSEPPE IACOMINO
  • LIVIA MALORNI
  • ANTONELLA VENEZIA
  • MARIA GRAZIA VOLPE

Lingua: inglese

Abstract: Titanium dioxide (TiO2) is enclosed in many consumer products including pharmaceuticals, cosmetics, and foods. TiO2 (E171) is daily ingested as mixed nano- and submicron-sized particles since it is approved as a white colorant in Europe in a wide variety of food products, Noteworthy, the relevant risk assessment has never been satisfactorily concluded and growing alarms for human hazards deriving from TiO2 exposure are incrementally reported. The objective of the present study was to establish conceivable mechanisms by which nano-sized TiO2 particles affect physiological function of the intestinal epithelium layer. The well-established Caco-2 cell line differentiated for 21 days on permeable supports was used as a predictive model of the human intestinal mucosa to identify the biological response triggered by TiO2 particles. Exposure to 42 µg/mL TiO2 nanoparticles disrupted the tight junctions-permeability barrier with a prompt effect detectable after 4h incubation time and wide effects on barrier integrity at 24h. Transport and ultrastructural localization of TiO2 nanoparticles were determined by ICP-OES, TEM and ESI/EELS analysis, respectively. Nano-sized particles were efficiently internalized and preferentially entrapped by Caco-2 monolayers. Storage of TiO2 nanoparticles inside the cells affected enterocytes viability and triggered the production of pro-inflammatory cytokines, including TNF-? and IL-8. Taken together these data indicate that nano-sized TiO2 particles exert detrimental effects on the intestinal epithelium layer.

Lingua abstract: inglese

Rivista:

Food research international Elsevier Applied Science on behalf of the Canadian Institute of Food Science and Technology,
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0963-9969

DOI: 10.1016/j.foodres.2018.10.041

Referee: Sì: Internazionale

Stato della pubblicazione: Postprint

Parole chiave:

  • Intestinal permeability
  • Toxicity
  • Nanomaterials
  • Food additives
  • Titanium dioxide
  • P25 TiO2

Strutture CNR:

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