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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2016

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Investigation of factors affecting aerobic and respiratory growth in the oxygen-tolerant strain Lactobacillus casei N87

Anno di pubblicazione: 2016

Formato: Elettronico

Autori: Ianniello R.G.; Zotta T.; Matera A.; Genovese F.; Parente E.; Ricciardi A.

Affiliazioni autori: Scuola di Scienze Agrarie, Forestali, Alimentari e Ambientali, Università degli Studi della Basilicata, Potenza, , Italy; Istituto di Scienze dell'Alimentazione-CNR, Avellino, , , Italy; Istituto di Scienze dell'Alimentazione-CNR, Avellino, , , Italy; Dipartimento di Scienze, Università degli Studi della Basilicata, Potenza, , Italy

Autori CNR:

  • TERESA ZOTTA

Lingua: inglese

Abstract: Aerobic and respiratory cultivations provide benefits for some lactic acid bacteria (LAB). Growth, metabolites, enzymatic activities (lactate dehydrogenase; pyruvate and NADH oxidases, NADH peroxidase; catalase), antioxidant capability and stress tolerance of Lactobacillus casei N87 were evaluated in anaerobic, aerobic and respiratory (aerobiosis with heme and menaquinone supplementation) batch cultivations with different dissolved oxygen (DO) concentrations. The expression of pox (pyruvate oxidase) and cydABCD operon (cytochrome bd oxidase complex) was quantified by quantitative Real Time polymerase chain reaction. Respiration increased biomass production compared to anaerobiosis and unsupplemented aerobiosis, and altered the central metabolism rerouting pyruvate away from lactate accumulation. All enzymatic activities, except lactate dehydrogenase, were higher in respiratory cultures, while unsupplemented aerobiosis with 60% of DO promoted H2O2 and free radical accumulation. Respiration improved the survival to oxidative and freeze-drying stresses, while significant numbers of dead, damaged and viable but not cultivable cells were found in unsupplemented aerobic cultures (60% DO). Analysis of gene expression suggested that the activation of aerobic and respiratory pathways occurred during the exponential growth phase, and that O2 and hemin induced, respectively, the transcription of pox and cydABCD genes. Respiratory cultivation might be a natural strategy to improve functional and technological properties of L. casei. © 2016 Ianniello et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Lingua abstract: inglese

Pagine da: e0164065

Rivista:

PloS one Public Library of Science
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 1932-6203

Numero volume: 11

DOI: 10.1371/journal.pone.0164065

Referee: Sì: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: Scopus [2-s2.0-84994030189]

Parole chiave:

  • Lactobacillus casei
  • respiratory growth
  • stress response
  • gene expression

URL: http://www.scopus.com/record/display.url?eid=2-s2.0-84994030189&origin=inward

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