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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2017

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Biochemical and Molecular Study of Carpobrotus edulis Bioactive Properties and Their Effects on Dugesia sicula (Turbellaria, Tricladida) Regeneration

Anno di pubblicazione: 2017

Formato: Elettronico

Autori: Meddeb E.; Charni M.; Ghazouani T.; Cozzolino A.; Fratianni F.; Raboudi F.; Nazzaro F.; Fattouch S.

Affiliazioni autori: Laboratory of Protein Engineering and Bioactive-Molecules (LIP-MB), National Institute of Applied Sciences and Technology (INSAT), University of Carthage, Zone Urbaine Nord, Tunis, 1080, , , Tunisia; Laboratory of Protein Engineering and Bioactive-Molecules (LIP-MB), National Institute of Applied Sciences and Technology (INSAT), University of Carthage, Zone Urbaine Nord, Tunis, 1080, , , Tunisia; Faculty of Sciences of Bizerte, University of Carthage, Tunis, , Tunisia; Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, , , Tunisia; Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis, , , Tunisia; College of Sciences and Humanities of Dawadmi, Shaqra University, Shangra, , Saudi Arabia; Laboratory of Biotechnology and Food Safety, Istituto di Scienze dell'Alimentazione, ISA-CNR, Avellino, , , Italy; Laboratory of Biotechnology and Food Safety, Istituto di Scienze dell'Alimentazione, ISA-CNR, Avellino, , , Italy; ISAJC, Bir El Bey, University of Tunis, Tunis, , Tunisia

Autori CNR:

  • FLORINDA FRATIANNI
  • FILOMENA NAZZARO

Lingua: inglese

Abstract: The traditional medicinal properties of Carpobrotus edulis are well recognized, particularly in Tunisia where it is used for wound healing. Thus, in this study, biochemical and molecular properties of its leaves' bioactive aqueous-acetone extract were investigated. The total phenolic content (TPC) of the extract was estimated to be 1845mg/100g of fresh matter (FM). The qualitative and quantitative polyphenolic profile was determined by ultra performance liquid chromatography with diode array detection (UPLC-DAD) and showed that chlorogenic acid was the major compound (43.7%). The extract exhibits potent antioxidant capacities with IC50=56.19 and 58.91?g/ml, as accessed via the anionic DPPH and cationic ABTS radical scavenging assays, respectively. The extract has high antibacterial properties, especially against the Gram+ Staphylococcus aureus and Bacillus cereus strains. To investigate the extract effect on regeneration, the flatworm Dugesia sicula Lepori, 1948, was used as a model. The macroscopic analysis of planarian cultures in ordinary medium containing phenolic extract at non-toxic concentrations illustrated that the extract caused morphological changes. Additionally, the molecular study through the fluorescence-activated cell sorting (FACS) technique showed that C. edulis polyphenols can harm the stem cells' development. These results emphasize the ecotoxicological impact of phenolic rejections in the environment on flatworms' physiology.

Lingua abstract: inglese

Pagine da: 1131

Pagine a: 1143

Rivista:

Applied biochemistry and biotechnology Humana Press,
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0273-2289

Numero volume: 182

DOI: 10.1007/s12010-016-2387-y

Referee: S: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: Scopus [2-s2.0-85008425590]

Parole chiave:

  • vegetal extracts
  • antimicrobial
  • citotoxicity

URL: http://www.scopus.com/record/display.url?eid=2-s2.0-85008425590&origin=inward

Strutture CNR:

 
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