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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2016

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Extensively hydrolyzed casein formula alone or with L. rhamnosus GG reduces ?-lactoglobulin sensitization in mice

Anno di pubblicazione: 2016

Autori: Aitoro R, Simeoli R, Amoroso A, Paparo L, Nocerino R, Pirozzi C, Di Costanzo M, Meli R, De Caro C, Picariello G, Mamone G, Calignano A, Nagler CR, Berni Canani R

Affiliazioni autori: Departments of Translational Medical Science; Pharmacy, University of Naples 'Federico II', Naples, Italy; Institute of Food Sciences, CNR, Avellino, Italy; Committee on Immunology and Departments of Pathology, University of Chicago, Chicago, IL, USA; European Laboratory for The Investigation of Food Induced Diseases; CEINGE Advanced Biotechnologies, University of Naples 'Federico II', Naples, Italy

Autori CNR:

  • GIANFRANCO MAMONE
  • GIANLUCA PICARIELLO

Lingua: inglese

Abstract: Background: Extensively hydrolyzed casein formula (EHCF) has been proposed for the prevention and is commonly used for the treatment of cow's milk allergy (CMA). The addition of the probiotic Lactobacillus rhamnosus GG (LGG) to EHCF may induce faster acquisition of tolerance to cow's milk. The mechanisms underlying this effect are largely unexplored. We investigated the effects of EHCF alone or in combination with LGG on b-lactoglobulin (BLG) sensitization in mice. Methods: Three-week-old C3H/HeOuJ mice were sensitized by oral administration of BLG using cholera toxin as adjuvant at weekly intervals for 5 weeks (sensitization period). Two experimental phases were conducted: (i) EHCF or EHCF+LGG given daily, starting 2 weeks before the sensitization period and then given daily for 5 weeks and (ii) EHCF or EHCF+LGG given daily for 4 weeks, starting 1 week after the sensitization period. Diet free of cow's milk protein was used as control. Acute allergic skin response, anaphylactic symptom score, body temperature, intestinal permeability, anti-BLG serum IgE, and interleukin (IL)-4, IL-5, IL-10, IL-13, IFN-c mRNA expression were analyzed. Peptide fractions of EHCF were characterized by reversedphase (RP)-HPLC, MALDI-TOF mass spectrometry, and nano-HPLC/ESI-MS/MS. Results: Extensively hydrolyzed casein formula administration before or after BLGinduced sensitization significantly reduced acute allergic skin reaction, anaphylactic symptom score, body temperature decrease, intestinal permeability increase, IL-4, IL-5, IL-13, and anti-BLG IgE production. EHCF increased expression of IFN-c and IL-10. Many of these effects were significantly enhanced by LGG supplementation. The peptide panels were similar between the two study formulas and contained sequences that could have immunoregulatory activities. Conclusions: The data support dietary intervention with EHCF for CMA prevention and treatment through a favorable immunomodulatory action. The observed effects are significantly enhanced by LGG supplementation.

Lingua abstract: inglese

Pagine da: 230

Pagine a: 237

Rivista:

Pediatric allergy and immunology Blackwell
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1399-3038

Numero volume: 28

Numero fascicolo: 3

DOI: 10.1111/pai.12687

Referee: Sė: Internazionale

Stato della pubblicazione: Published version

Parole chiave:

  • bovine casein; food allergy; hypoallergenic formula; intestinal permeability; probiotics

Strutture CNR:

 
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