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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2018

Contributo in rivista

Tipo: Articolo in rivista

Titolo: A proteometabolomic study of Actinidia deliciosa fruit development

Anno di pubblicazione: 2018

Formato: Elettronico Cartaceo

Autori: Anna Maria Salzano, Anatoly Sobolev, Virginia Carbone, Milena Petriccione, Giovanni Renzone, Donatella Capitani, Monica Vitale, Paola Minasi, Maria Silvia Pasquariello, Gianfranco Novi, Nicola Zambrano, Marco Scortichini, Luisa Mannina, Andrea Scaloni

Affiliazioni autori: Proteomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Naples, Italy; Magnetic Resonance Laboratory "Annalaura Segre", Institute of Chemical Methodologies, National Research Council, 00015, Monterotondo, Rome, Italy; Institute of Food Sciences, National Research Council, 83100 Avellino, Italy; Centro di Ricerca per Olivicoltura, Frutticoltura e Agrumicoltura, Consiglio per la Ricerca in Agricoltura e l'Analisi dell'Economia Agraria, 81100 Caserta, Italy; Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli "Federico II", 80131 Naples, Italy; CEINGE Biotecnologie Avanzate, 80145 Naples, Italy; Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, 00185 Rome, Italy

Autori CNR:

  • DONATELLA CAPITANI
  • VIRGINIA CARBONE
  • LUISA MANNINA
  • PAOLA MINASI
  • GIANFRANCO NOVI
  • GIOVANNI RENZONE
  • ANNA MARIA SALZANO
  • ANDREA SCALONI
  • ANATOLY SOBOLEV
  • MONICA VITALE

Lingua: inglese

Abstract: Green-fleshed kiwifruit is worldwide appreciated for its flavor and macronutrients that are related to its physiological development. Fruit ripe stage for harvesting does not correspond to an optimal edible condition due to firmness/acidity pome characteristics; this is overcome with postharvest fruit processing. To describe the metabolic pathways/molecular effectors underlying Actinidia deliciosa cv. Hayward pome physiological development, kiwifruits were harvested at four growth stages (from fruit set to physiological maturity), and corresponding outer endocarp samples were analysed for metabolite and protein content. Combined NMR/LC-UV/ESI-IT-MSn procedures quantified 46 metabolites at these developmental stages; similarly, integrated 2D-DIGE/nLC-ESI-LIT-MS/MS analyses described corresponding proteomic changes. Quantitative protein dynamics showed that components related to disease/defense, protein destination/storage, metabolism, energy and cell structure functions were highly affected at specific moments of fruit development, suggesting a rationale to pomological and metabolite content characteristics at those times. Bioinformatic interaction prediction revealed a main network of differentially represented proteins, which may control metabolic changes in developing kiwifruit. Main pome allergens were also quantified, demonstrating their highest levels at the mature stage. By aligning kiwifruit development to a proteometabolomic representation, this investigation integrates previous metabolic observations and provides a reference framework for further physiological/nutritional studies, also allowing cross comparison among crop species.

Lingua abstract: inglese

Pagine da: 11

Pagine a: 24

Pagine totali: 14

Rivista:

Journal of proteomics Elsevier
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 1874-3919

Numero volume: 172

DOI: 10.1016/j.jprot.2017.11.004

Referee: Sì: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: PubMed [29133123]

Parole chiave:

  • Kiwifruit Pome development Proteomics Metabolomics Metabolites

URL: http://www.sciencedirect.com/science/article/pii/S1874391917303755?via%3Dihub

Data di accettazione: 08/11/2017

Strutture CNR:

 
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