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Contributo in rivista
Tipo: Articolo in rivista
Titolo: Differential representation of liver proteins in obese human subjects suggests novel biomarkers and promising targets for drug development in obesity.
Anno di pubblicazione: 2017
Formato: Elettronico Cartaceo
Autori: Caira, Simonetta; Iannelli, Antonio; Sciarrillo, Rosaria; Picariello, Gianluca; Renzone, Giovanni; Scaloni, Andrea; Addeo, Pietro
Affiliazioni autori: Proteomics and Mass Spectrometry Laboratory , ISPAAM, National Research Council , Naples , Italy. Département de Chirurgie Digestive , Centre Hospitalier Universitarie de Nice , Nice , France. Dipartimento di Scienze e Tecnologie , Università degli Studi del Sannio , Benevento , Italy. Institute of Food Sciences, National Research Council , Avellino , Italy. Service de Chirurgie Hépatique, Pancréatique, Biliaire et Transplantation, Pôle des Pathologies Digestives, Hépatiques et de la Transplantation, Hôpital de Hautepierre , Université de Strasbourg, Hôpitaux Universitaires de Strasbourg , Strasbourg , France.
Abstract: The proteome of liver biopsies from human obese (O) subjects has been compared to those of nonobese (NO) subjects using two-dimensional gel electrophoresis (2-DE). Differentially represented proteins were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS)-based peptide mass fingerprinting (PMF) and nanoflow-liquid chromatography coupled to electrospray-tandem mass spectrometry (nLC-ESI-MS/MS). Overall, 61 gene products common to all of the liver biopsies were identified within 65 spots, among which 25 ones were differently represented between O and NO subjects. In particular, over-representation of short-chain acyl-CoA dehydrogenase, Delta(3,5)-Delta(2,4)dienoyl-CoA isomerase, acetyl-CoA acetyltransferase, glyoxylate reductase/hydroxypyruvate reductase, fructose-biphosphate aldolase B, peroxiredoxin I, protein DJ-1, catalase, alpha- and beta-hemoglobin subunits, 3-mercaptopyruvate S-transferase, calreticulin, aminoacylase 1, phenazine biosynthesis-like domain-containing protein and a form of fatty acid-binding protein, together with downrepresentation of glutamate dehydrogenase, glutathione S-transferase A1, S-adenosylmethionine synthase 1A and a form of apolipoprotein A-I, was associated with the obesity condition. Some of these metabolic enzymes and antioxidant proteins have already been identified as putative diagnostic markers of liver dysfunction in animal models of steatosis or obesity, suggesting additional investigations on their role in these syndromes. Their differential representation in human liver was suggestive of their consideration as obesity human biomarkers and for the development of novel antiobesity drugs.
Lingua abstract: inglese
Pagine da: 672
Pagine a: 682
Pagine totali: 11
Journal of enzyme inhibition and medicinal chemistry
Taylor & Francis,
Numero volume: 32
Numero fascicolo: 1
Referee: Sì: Internazionale
Stato della pubblicazione: Published version
Data di accettazione: 01/02/2017
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