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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2016

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Pb2+ Effects on Growth, Lipids, and Protein and DNA Profiles of the Thermophilic Bacterium Thermus thermophilus

Anno di pubblicazione: 2016

Formato: Elettronico

Autori: Barbara Nicolaus, Annarita Poli, Paola Di Donato, Ida Romano, Giusi Laezza, Alessia Gioiello, Sergio Ulgiati, Florinda Fratianni, Filomena Nazzaro, Pierangelo Orlando, Stefano Dumontet

Affiliazioni autori: ICB-CNR, Università Parthenope, ISA-CNR

Autori CNR:

  • PAOLA DI DONATO
  • FLORINDA FRATIANNI
  • ALESSIA GIOIELLO
  • FILOMENA NAZZARO
  • BARBARA NICOLAUS
  • PIERANGELO ORLANDO
  • ANNARITA POLI
  • IDA ROMANO

Lingua: inglese

Abstract: Extremophiles are organisms able to thrive in extreme environmental conditions and some of them show the ability to survive high doses of heavy metals thanks to defensive mechanisms provided by primary and secondary metabolic products, i.e., extremolytes, lipids, and extremozymes. This is why there is a growing scientific and industrial interest in the use of thermophilic bacteria in a host of tasks, from the environmental detoxification of heavy metal to industrial activities, such as bio-machining and bio-metallurgy. In this work Thermus thermophilus was challenged against increasing Pb2+ concentrations spanning from 0 to 300 ppm in order to ascertain the sensitiveness of this bacteria to the Pb environmental pollution and to give an insight on its heavy metal resistance mechanisms. Analysis of growth parameters, enzyme activities, protein profiles, and lipid membrane modifications were carried out. In addition, genotyping analysis of bacteria grown in the presence of Pb2+, using random amplified polymorphic DNA-PCR and DNA melting evaluation, were also performed. A better knowledge of the response of thermophilic bacteria to the different pollutants, as heavy metals, is necessary for optimizing their use in remediation or decontamination processes

Lingua abstract: inglese

Rivista:

Microorganisms Molecular Diversity Preservation International
Paese di pubblicazione:
Lingua: inglese
ISSN: 2076-2607

Numero volume: 4

DOI: 10.3390/microorganisms4040045

Referee: Sì: Internazionale

Stato della pubblicazione: Published version

Parole chiave:

  • heavy metals
  • Thermus thermophilus
  • lead effects on lipid
  • DNA and protein

Strutture CNR:

 
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