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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2016

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Polymorphism of the phosphoserine phosphatase gene in Streptococcus thermophilus and its potential use for typing and monitoring of population diversity.

Anno di pubblicazione: 2016

Formato: Elettronico Cartaceo

Autori: Annamaria Ricciardi, Francesca De Filippis, Teresa Zotta, Angelo Facchiano, Danilo Ercolini, Eugenio Parente

Affiliazioni autori: a Scuola di Scienze Agrarie, Forestali, Alimentari ed Ambientali, Università degli Studi della Basilicata, Potenza, Italy b Department of Agricultural Sciences, Division of Microbiology, University of Naples Federico II, Portici, Italy c Istituto di Scienze dell'Alimentazione, CNR, Avellino, Italy d Dipartimento di Scienze, Università degli Studi della Basilicata, Potenza, Italy

Autori CNR:

  • ANGELO FACCHIANO
  • TERESA ZOTTA

Lingua: inglese

Abstract: The phosphoserine phosphatase gene (serB) of Streptococcus thermophilus is the most polymorphic gene among those used in Multilocus Sequence Typing schemes for this species and has been used for both genotyping of isolates and for evaluation of population diversity. However, the information on the potential of this gene as a marker for diversity in S. thermophilus species is still fragmentary. In this study, we evaluated serB nucleotide polymorphism and its potential impact on protein structure using data from traditional sequencing. In addition we evaluated the ability of serB targeted high-throughput sequencing in studying the diversity of S. thermophilus populations in cheese and starter cultures. Data based on traditional cultivation based techniques and sequencing provided evidence that the distribution of serB alleles varies significantly in some environments (commercial starter cultures, traditional starter cultures, cheese). Mutations had relatively little impact on predicted protein structure and were not found in domains that are predicted to be important for its functionality. Cultivation independent, serB targeted high-throughput sequencing provided evidence for significantly different alleles distribution in different cheese types and detected fluctuations in alleles abundance in a mixed strain starter reproduced by backslopping. Notwithstanding some shortcomings of this method that are discussed here, the cultivation independent approach appears to be more sensitive than cultivation based approaches based on isolation and traditional sequencing.

Lingua abstract: inglese

Pagine da: 138

Pagine a: 147

Rivista:

International journal of food microbiology Elsevier
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 0168-1605

Numero volume: 236

Numero fascicolo: 7

DOI: 10.1016/j.ijfoodmicro.2016.07.031

Referee: Sì: Internazionale

Stato della pubblicazione: Published version

Parole chiave:

  • Streptococcus thermophilus; Microdiversity; Phosphoserine phosphatase; High-throughput sequencing

Strutture CNR:

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