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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2016

Contributo in rivista

Tipo: Articolo in rivista

Titolo: New antimicrobial peptides against foodborne pathogens: From in silico design to experimental evidence

Anno di pubblicazione: 2016

Formato: Elettronico Cartaceo

Autori: Palmieri, Gianna; Balestrieri, Marco; Proroga, Yolande T R; Falcigno, Lucia; Facchiano, Angelo; Riccio, Alessia; Capuano, Federico; Marrone, Raffaele; Neglia, Gianluca; Anastasio, Aniello

Affiliazioni autori: Consiglio Nazionale delle Ricerche; Istituto Zooprofilattico Sperimentale del Mezzogiorno; Universita degli Studi di Napoli Federico II; Consiglio Nazionale delle Ricerche; Universita degli Studi di Napoli Federico II

Autori CNR:

  • MARCO BALESTRIERI
  • ANGELO FACCHIANO
  • GIANNA PALMIERI

Lingua: inglese

Abstract: Recently there has been growing interest in the discovery of new antimicrobial agents to increase safety and shelf-life of food products. Here, we developed an innovative approach by introducing the concept that mitochondrial targeting peptides (MTP) can interact and disrupt bacterial membranes, acting as antimicrobial agents. As proof-of-principle, we used a multidisciplinary strategy by combining in silico predictions, docking simulations and antimicrobial assays, to identify two peptides, MTP1 and MTP2, which were structurally and functionally characterized. Both compounds appeared effective against Listeria monocytogenes, one of the most important foodborne pathogens. Specifically, a significant bactericidal activity was evidenced with EC50 values of 16.8 ± 1.2 ?M for MTP1 and 109 ± 7.0 ?M for MTP2. Finally, NMR structure determinations suggested that MTP1 would be oriented into the membrane bilayer, while the molecular shape of MTP2 could indicate porin-mediated antimicrobial mechanisms, as predicted using molecular docking analysis. Therefore, MTPs represent alternative sources to design new potential bio-preservatives.

Lingua abstract: inglese

Pagine da: 546

Pagine a: 554

Rivista:

Food chemistry Applied Science Publishers.
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0308-8146

Numero volume: 211

DOI: 10.1016/j.foodchem.2016.05.100

Referee: Sì: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: Scopus [2-s2.0-84969699705]

Parole chiave:

  • Antimicrobial peptide
  • Food bio-preservatives
  • Listeria monocytogenes
  • Mitochondrial-targeted peptide
  • Structural analysis

URL: http://www.scopus.com/record/display.url?eid=2-s2.0-84969699705&origin=inward

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