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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2016

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Celiac disease: role of intestinal compartments in the mucosal immune response

Anno di pubblicazione: 2016

Formato: Elettronico Cartaceo

Autori: Gaetano Iaquinto1, Vera Rotondi Aufiero2, Giuseppe Iacomino2, Giuseppe Mazzarella2, Michele Schettino3 and Armando Masucci3

Affiliazioni autori: 1Gastroenterolgy, Santa Rita Hospital and N. Taccone Foundation, Atripalda (AV), Italy 2Institute of Food Sciences-CNR, Avellino, Italy 3Gastroenterology and Digestive Endoscopy Service, San G. Moscati Hospital, Avellino, Italy Abstract

Autori CNR:

  • GIUSEPPE IACOMINO

Lingua: inglese

Abstract: Background Different approaches have been used to study the pattern of cytokines in celiac disease (CD). Laser capture microdissection (LCM) is a powerful tool for the isolation of specific tissue compartments. We aimed to investigate the mucosal immune response that takes place in different intestinal compartments of CD patients, dissected by LCM, analyzing cytokine expression profile Methods Frozen section of jejunum were obtained from 15 untreated CD and 15 control. Surface epithelium and lamina propria compartment were isolated by LCM. RNA from each LCM sample was extracted and, after a retrotranscription step, messenger RNA (mRNA) levels for MxA, IL-15, TNF-?, IFN-?, IL-17?, IL-21, IL-10 and TGF-? were determined by quantitative reverse transcriptase-PCR. Results Increased gene expression levels of MxA, IL-15, TNF-?, IL-10 and TGF-? in the surface epithelium of untreated CD with respect to control. Furthermore, all the cytokines investigated were upregulated in the lamina propria of untreated CD as compared to control. Within the untreated CD group the expression of IL-15 was higher, in the surface epithelium than in the lamina propria, whereas the expression of IL-17 and IL-21 were higher in the lamina propria than in the surface epithelium. Finally, high levels of IL-10 and TGF-? were detected in both compartments of untreated CD biopsies. Conclusion In CD, surface epithelium and lamina propria compartments, play a prominent role in determining innate and adaptive immunity, respectively. Conversely, surface epithelium and lamina propria produce high levels of anti-inflammatory cytokines, suggesting that both compartments are involved in the immunoregulatory response.

Lingua abstract: inglese

Pagine da: 921.10-921.10

Pagine totali: 1

Rivista:

The FASEB journal Federation of American Societies for Experimental Biology,
Paese di pubblicazione: Stati Uniti d'America
Lingua: inglese
ISSN: 0892-6638

Numero volume: 30

Numero fascicolo: 1

Stato della pubblicazione: Published version

Parole chiave:

  • Celiac disease
  • mucosal immune response
  • LCM
  • transcriptional response

Strutture CNR:

 
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