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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2016

Contributo in rivista

Tipo: Articolo in rivista

Titolo: DNA-HMGB1 interaction: The nuclear aggregates of polyamine mediation

Anno di pubblicazione: 2016

Formato: Elettronico Cartaceo

Autori: Giuseppe Iacomino a, Gianluca Picariello a, Francesca Sbrana b, Roberto Raiteri b, c, Luciano D'Agostino a

Affiliazioni autori: a Istituto di Scienze dell'Alimentazione - CNR, Via Roma 64 - 83100, Avellino, Italy b Istituto di Biofisica - CNR, Via De Marini 6 - 16149, Genova, Italy c Dipartimento di Informatica, Bioingegneria, Robotica ed Ingegneria dei Sistemi - Università degli Studi di Genova, Via All'Opera Pia 13 - 16145, Genova, Italy

Autori CNR:

  • LUCIANO D'AGOSTINO
  • GIUSEPPE IACOMINO
  • GIANLUCA PICARIELLO

Lingua: inglese

Abstract: Nuclear aggregates of polyamines (NAPs) are supramolecular compounds generated by the self-assembly of protonated nuclear polyamines (spermine, spermidine and putrescine) and phosphate ions. In the presence of genomic DNA, the hierarchical process of self-structuring ultimately produces nanotube-like polymers that envelop the double helix. Because of their modular nature and their aggregation-disaggregation dynamics, NAPs confer plasticity and flexibility to DNA. Through the disposition of charges, NAPs also enable a bidirectional stream of information between the genome and interacting moieties. High mobility group (HMG) B1 is a non-histone chromosomal protein that binds to DNA and that influences multiple nuclear processes. Because genomic DNA binds to either NAPs or HMGB1 protein, we explored the ability of in vitro self-assembled NAPs (ivNAPs) to mediate the DNA-HMGB1 interaction. To this end, we structured DNA-NAPs-HMGB1 and DNA-HMGB1-NAPs ternary complexes in vitro through opportune sequential incubations. Mobility shift electrophoresis and atomic force microscopy showed that the DNA-ivNAPs-HGMB1 complex had conformational assets supposedly more suitable those of the DNA-HGMB1-ivNAPs to comply with the physiological and functional requirements of DNA. Our findings indicated that ivNAPs act as mediators of the DNA-HMGB1 interaction.

Lingua abstract: inglese

Rivista:

Biochimica et biophysica acta. Proteins and proteomics Elsevier
Paese di pubblicazione: Paesi Bassi
Lingua: inglese
ISSN: 1570-9639

DOI: 10.1016/j.bbapap.2016.07.006

Referee: Sì: Internazionale

Stato della pubblicazione: Preprint

Parole chiave:

  • Nuclear aggregates of polyamines
  • HMGB1
  • EMSA
  • Atomic force microscopy
  • DNA conformational transitions
  • Supramolecular nanotubes

Data di accettazione: 19/07/2016

Strutture CNR:

 
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