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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2016

Contributo in rivista

Tipo: Articolo in rivista

Titolo: A Shear horizontal surface acoustic wave biosensor for a rapid and specific detection of d-serine

Anno di pubblicazione: 2016

Formato: Elettronico Cartaceo

Autori: Di Pietrantonio F.; Benetti M.; Cannata D.; Verona E.; Girasole M.; Fosca M.; Dinarelli S.; Staiano M.; Marzullo V.M.; Capo A.; Varriale A.; D'Auria S.

Affiliazioni autori: Institute of Acoustics and Sensors O. M. Corbino, CNR, Rome, Italy; Institute for Photonics and Nanotechnologies, CNR, Rome, Italy; Istituto di Struttura della Materia, CNR, Rome, Italy; Institute of Food Science, CNR, Avellino, Italy; Institute of Protein Biochemistry, CNR, Napoli, Italy

Autori CNR:

  • MASSIMILIANO BENETTI
  • DOMENICO CANNATA'
  • ALESSANDRO CAPO
  • SABATO D'AURIA
  • FABIO DI PIETRANTONIO
  • SIMONE DINARELLI
  • MARCO FOSCA
  • MARCO GIRASOLE
  • VINCENZO MANUEL MARZULLO
  • MARIA STAIANO
  • ANTONIO VARRIALE
  • ENRICO VERONA

Lingua: inglese

Abstract: d-serine is an amino acid that has an important role in the regulation of N-methyl-d-aspartate (NMDA) signalling in the central nervous system. In fact, it acts as an endogenous co-agonist molecule for the NMDA receptor activation. Since an abnormal body level of d-amino acids is usually associated to different neurological diseases, it appears evident that it is of high interest to develop a fast and specific biosensor for the determination of d-serine. In this work, we report on the design and development of a surface acoustic wave (SAW) biosensor to detect the level of d-serine. The SAW-biosensor is based on the utilization of an inactivated form of the d-serine dehydratase isolated from Saccharomyces cerevisiae, a biomolecule that is able to specifically bind d-serine. In particular, we produced a PLP-reduced d-serine dehydratase biomolecule that was still able to bind d-serine and not to transform it into the product. The binding event between the "inactivated" d-serine dehydratase and d-serine was monitored by SAW delay-lines.

Lingua abstract: inglese

Pagine da: 1

Pagine a: 6

Rivista:

Sensors and actuators. B, Chemical Elsevier Sequoia
Paese di pubblicazione: Svizzera
Lingua: inglese
ISSN: 0925-4005

Numero volume: 226

DOI: 10.1016/j.snb.2015.11.099

Stato della pubblicazione: Published version

Indicizzato da: Scopus [2-s2.0-84949501572]

Parole chiave:

  • d-serine dehydratase
  • d-serine detection
  • Surface acoustic wave biosensor

URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-84949501572&partnerID=q2rCbXpz

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