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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2016

Contributo in rivista

Tipo: Articolo in rivista

Titolo: A novel fluorescence polarization assay for determination of penicillin G in milk

Anno di pubblicazione: 2016

Formato: Cartaceo

Autori: Pennacchio A.; Varriale A.; Scala A.; Marzullo V.M.; Staiano M.; D'Auria S.

Affiliazioni autori: Laboratory for Molecular Sensing, Institute of Protein Biochemistry CNR, Via P. Castellino 111, Naples, 80131, Italy; Institute of Food Science, CNR, Via Roma 64, Avellino, 83100, Italy

Autori CNR:

  • SABATO D'AURIA
  • VINCENZO MANUEL MARZULLO
  • ANNA PENNACCHIO
  • MARIA STAIANO
  • ANTONIO VARRIALE

Lingua: inglese

Abstract: Detection of penicillin G in milk is of interest because of the wide use of this antibiotic in livestock. Current analytical methods used to quantify the penicillin G in milk are based on HPLC, mass spectrometry and electrophoresis. These methodologies are time-consuming and require trained personnel. In addition, it is not easy to envisage the development of a portable device for in situ analyses based on these methods. We present a novel sensing approach for detecting the presence of penicillin G in milk. The proposed method is based penicillin G conjugate labeled with red-emitting dye with properly produced anti-penicillin G antibodies. The results obtained suggest our method could be applied directly in milk without interference from other substances. The limit of detection of the method was 1.0 nmol/L, which is much less than the required MRL in EU regulations (12.0 nmol/L).

Lingua abstract: inglese

Pagine da: 381

Pagine a: 385

Rivista:

Food chemistry Applied Science Publishers.
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 0308-8146

Numero volume: 190

DOI: 10.1016/j.foodchem.2015.05.127

Stato della pubblicazione: Published version

Indicizzato da: Scopus [2-s2.0-84930959855]

Parole chiave:

  • Antibody
  • Biosensors
  • Fluorescence
  • Milk
  • Penicillin G

URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-84930959855&partnerID=q2rCbXpz

Strutture CNR:

 
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