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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in atti di convegno anno 2015

Contributo in atti di convegno

Tipo: Abstract in atti di convegno

Titolo: T. monococcum omega-gliadin protects against gliadin-induced toxicity in differentiated Caco-2 cell

Anno di pubblicazione: 2015

Formato: Cartaceo

Autori: Giuseppe Iacomino, Ilenia Gavitone, Luigia Di Stasio, Olga Fierro, Antonella Venezia, Gianfranco Mamone.

Affiliazioni autori: ISA-CNR

Autori CNR:

  • GIUSEPPE IACOMINO

Lingua: inglese

Abstract: AIMS: Celiac Disease (CD) is the result of the interaction between a series of composite mechanisms involving genetic and environmental factors. Gliadin proteins are the trigger factor of CD symptoms. Gliadins are alcohol-soluble components representing about half a gluten protein and are traditionally subdivided into ?/?-, ?-, and ?-fractions. Because of high percentage of proline residues, gliadins are resistant to gastrointestinal digestion so that long gluten fragments can reach high concentration levels in the gut stimulating either adaptive or innate immune responses [1]. Beside immunodominant and toxic peptides, wheat proteins can also contain minor variations in the amino acid sequence of epitopes involved in the CD process capable to interfere with the gliadin-induced toxicity that were addressed in the present study. METHODS: We identified an ?-gliadin peptide which was resistant to in vitro gastrointestinal digestion from diploid wheat -Triticum monococcum, ID331- cultivar. This peptide (QSFPQQPQRPQPFPQQPEQ sequence) includes a protective sequence amino acid (QQPQRPQPF), which is known to preclude the stimulation of CD-specific mucosal T cells by gliadin proteins [2]. The ?-gliadin peptide was synthetized, incubated on differentiated Caco-2 cell together with hydrolyzed gliadin sample from Triticum aestivum cultivars, and resulting effects were evaluated by assessing the relative cytotoxicity and cytoskeleton reorganization. RESULTS: Co-administration of ?-gliadin peptide significantly prevents the in vitro gliadin-induced epithelial toxicity (evaluated by ATP cytotoxicity assay) and avoids cytoskeleton reorganization (assessed by immunofluorescent staining of F-actin). CONCLUSION: Outcomes may represent a new challenge for the development of innovative approaches to reduce gluten toxicity in gluten related disorders. [1] Mamone et al, Expert Rev Proteomics. 2011,8: 95-115 [2] De Vita et al, Journal of Cereal Science 2012, 55: 234-242

Referee: Sì: Internazionale

Stato della pubblicazione: Published version

Parole chiave:

  • Celiac disease
  • Triticum monococcum
  • ID331
  • gliadin-induced toxicity

Congresso nome: 16th International Celiac Disease Symposium ICDS 2015

Congresso luogo: Prague

Congresso data: June, 21-24, 2015

Congresso rilevanza: Internazionale

Congresso relazione: Contributo

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