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Istituto di scienza dell'alimentazione

Torna all'elenco Contributi in rivista anno 2015

Contributo in rivista

Tipo: Articolo in rivista

Titolo: Biochemical analysis of respiratory metabolism in the heterofermentative Lactobacillus spicheri and Lactobacillus reuteri

Anno di pubblicazione: 2015

Formato: Elettronico

Autori: Ianniello R.G.; Zheng J.; Zotta T.; Ricciardi A.; Ganzle M.G.

Affiliazioni autori: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada; Scuola di Scienze Agrarie, Forestali, Alimentari e Ambientali, Università degli Studi della Basilicata, Potenza, Italy; State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, China; Istituto di Scienze dell'Alimentazione-CNR, Avellino, Italy

Autori CNR:

  • TERESA ZOTTA

Lingua: inglese

Abstract: Aims: This study evaluated the aerobic and respiratory metabolism in Lactobacillus reuteri and Lactobacillus spicheri, two heterofermentative species used in sourdough fermentation. Methods and Results: In silico genome analysis, production of metabolites and gene expression of pyruvate oxidase, pyruvate dehydrogenase and cytochrome oxidase were assessed in anaerobic and aerobic cultures of Lact. reuteri and Lact. spicheri. Respiring homofermentative Lactobacillus casei N87 and Lact. rhamnosus N132 were used for comparison. Aerobiosis and respiration increased the biomass production of heterofermentative strains compared to anaerobic cultivation. Respiration led to acetoin production by Lact. rhamnosus and Lact. casei, but not in heterofermentative strains, in which lactate and acetate were the major end-products. Lactobacillus spicheri LP38 showed the highest oxygen uptake. Pyruvate oxidase, respiratory cytochromes, NADH oxidase and NADH peroxidase were present in the genome of Lact. spicheri LP38. Both Lact. spicheri LP38 and Lact. rhamnosus N132 overexpressed pox in aerobic cultures, while cydA was up-regulated only when haeme was supplied; pdh was repressed during aerobic growth. Conclusions: Aerobic and respiratory growth provided physiological and metabolic advantages also in heterofermentative lactobacilli. Significance and Impact of the Study: The exploitation of oxygen-tolerant phenotypes of Lact. spicheri may be useful for the development of improved starter cultures.

Lingua abstract: inglese

Pagine da: 763

Pagine a: 775

Rivista:

Journal of applied microbiology Blackwell Science.
Paese di pubblicazione: Regno Unito
Lingua: inglese
ISSN: 1365-2672

Numero volume: 119

Numero fascicolo: 3

DOI: 10.1111/jam.12853

Referee: Sì: Internazionale

Stato della pubblicazione: Published version

Indicizzato da: Scopus [2-s2.0-84939574585]

Parole chiave:

  • Genome analysis
  • Lactobacillus reuteri
  • Lactobacillus spicheri
  • Metabolism
  • Respiration

URL: http://www.scopus.com/inward/record.url?eid=2-s2.0-84939574585&partnerID=q2rCbXpz

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